Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4728563..4729060 Replicon chromosome
Accession NZ_CP071954
Organism Escherichia coli strain SB0258h1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J5X85_RS23010 Protein ID WP_001173343.1
Coordinates 4728563..4728736 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag J5X85_RS23015 Protein ID WP_000398619.1
Coordinates 4728764..4729060 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5X85_RS23000 (4725233) 4725233..4726690 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
J5X85_RS23005 (4726927) 4726927..4728444 + 1518 WP_001550472.1 lysine--tRNA ligase -
J5X85_RS23010 (4728563) 4728563..4728736 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J5X85_RS23015 (4728764) 4728764..4729060 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J5X85_RS23020 (4729289) 4729289..4729561 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
J5X85_RS23025 (4729573) 4729573..4729803 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
J5X85_RS23030 (4729983) 4729983..4731614 + 1632 WP_001216477.1 sensor histidine kinase -
J5X85_RS23035 (4731611) 4731611..4732330 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 4683677..4729060 45383


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T197180 WP_001173343.1 NZ_CP071954:c4728736-4728563 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T197180 NZ_CP071954:c4728736-4728563 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT197180 WP_000398619.1 NZ_CP071954:c4729060-4728764 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT197180 NZ_CP071954:c4729060-4728764 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References