Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3771031..3771649 Replicon chromosome
Accession NZ_CP071954
Organism Escherichia coli strain SB0258h1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J5X85_RS18235 Protein ID WP_001291435.1
Coordinates 3771431..3771649 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J5X85_RS18230 Protein ID WP_000344800.1
Coordinates 3771031..3771405 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5X85_RS18220 (3766120) 3766120..3767313 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J5X85_RS18225 (3767336) 3767336..3770485 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
J5X85_RS18230 (3771031) 3771031..3771405 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J5X85_RS18235 (3771431) 3771431..3771649 + 219 WP_001291435.1 HHA domain-containing protein Toxin
J5X85_RS18240 (3771821) 3771821..3772372 + 552 WP_060579167.1 maltose O-acetyltransferase -
J5X85_RS18245 (3772488) 3772488..3772958 + 471 WP_000136192.1 YlaC family protein -
J5X85_RS18250 (3773122) 3773122..3774672 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J5X85_RS18255 (3774714) 3774714..3775067 - 354 WP_001550741.1 DUF1428 family protein -
J5X85_RS18265 (3775446) 3775446..3775757 + 312 WP_000409911.1 MGMT family protein -
J5X85_RS18270 (3775788) 3775788..3776360 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T197174 WP_001291435.1 NZ_CP071954:3771431-3771649 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T197174 NZ_CP095766:c2937227-2936931 [Pseudomonas chengduensis]
ATGGAAAAGCGCAAACCCCATTTCAAGCTTGAGCTGGTGAAGCAGGCCGTAACCGAGCAGCGTTATCGCTTCACCCGCGT
AGCCTTGGAAGGGGGCGCCGAGCTGGGTATGGAGATGGCTGACATGCTGGCCGTTATCAGTGCCCTGAGCAGCCGCGACT
TTTTCAAGAGCATGACAACCTATGCGGATCATACTACCTGGCAGGACGTTTACCGACCCGACACCGAATTCGGGCAGGTC
TATCTGAAGTTCACGCTGGTGGCCGATCTGCTGATCGTTTCCTTCAAGGAGAAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT197174 WP_000344800.1 NZ_CP071954:3771031-3771405 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT197174 NZ_CP095766:c2936928-2936527 [Pseudomonas chengduensis]
ATGAAATGTCCAGTATGCGGCGGGGCGGAACTGGTTCACGACACCCGCGATATGCCCTTCACCTACAAGGGGCAGACCAC
CCAAATTACTGCAGTAACGGCTGACTGGTGCGATGCGTGCGGCGAGTCCCTGACTGGGCCGGCTGAGAGTGAGCGCGTTA
TGCGTGCCATGAACGAGTTTCGCCAGCAGGTGAACGCCCAGGACGGCAATCAGGAGCTTATTCGCAGCGTGCGTAAGCAG
TTGCGGTTGAGCCAACGTGAAGCGGCCGAGCTGTTCGGCGGTGGGCCGAATGCGTTTTCTCGTTACGAGCGAGGCAGCAC
AGAAGCACCGCAACCGCTGGTGCAGTTGTTCAAAATCTTGGGGCGACACCCGGAGTTGATCAACGAGCTGCGTGCTGGTT
GA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References