Detailed information of TA system TA197173

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3284611..3285316
• Replicon: chromosome
• Accession: NZ_CP071954
• Organism: Escherichia coli strain SB0258h1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: J5X85_RS15880
• Protein ID: WP_000539521.1
• Coordinates: 3284611..3284997 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J5X85_RS15885
• Protein ID: WP_001280945.1
• Coordinates: 3284987..3285316 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5X85_RS15860 (3280615)	3280615..3281241	+	627	WP_001295292.1	glutathione S-transferase GstB	-
J5X85_RS15865 (3281238)	3281238..3282353	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
J5X85_RS15870 (3282464)	3282464..3282847	-	384	WP_000497137.1	biofilm formation regulator BssR	-
J5X85_RS15875 (3283060)	3283060..3284385	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
J5X85_RS15880 (3284611)	3284611..3284997	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J5X85_RS15885 (3284987)	3284987..3285316	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
J5X85_RS15890 (3285386)	3285386..3286714	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
J5X85_RS15895 (3286722)	3286722..3289070	-	2349	WP_001550886.1	EAL domain-containing protein	-
J5X85_RS15900 (3289248)	3289248..3290159	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T197173 WP_000539521.1 NZ_CP071954:3284611-3284997 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T197173 NZ_CP095766:c937896-937600 [Pseudomonas chengduensis]
ATGGCTGAAATAGTCTGGACGAACACCGCCCTCGAGCAGCTCGACGATCTCGCGCACTACATCGCCCTCGACAAACCGGA
CGCCACGCGAACGCTGGTCAGGAGGGCCGTTGAAACGGTTTCACGGTTAGCAGACTTTCCCCTGTCGGGCCGTGTTCCGG
ATGAGCTTCCACACTCCGTGTACCGCGAAATCGTCGTCCCACCCTGCCGAATTTTCTACCGCTATACAGACACAACGGTT
TTCATCATCCACATCATGCGAGAGGAGCGCGTGCTGCGCGCCCACATGCTCGAGTGA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT197173 WP_001280945.1 NZ_CP071954:3284987-3285316 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT197173 NZ_CP095766:c938141-937884 [Pseudomonas chengduensis]
ATGAAAGTCGAGTTGGTCACCAACCTGAAGCGACAGGCCACCAAGATTCTGGCCGACCTTCACGAGTCCAAAGAACCTGT
ACTGATCACCGAGCATGGAATGCCTTCGGCTTATCTGGTGGATGTCGATGACTACCAGTTCATGCAGAGGCGTCTTGCCC
TTCTGGATGCCCTGGCTCGCGGAGAACGTGCCGTAATCGAGGGCAACACCCAGAACCATGAGCAGGCCAAGGAACGGCTG
AGCAAATGGCTGAAATAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6