Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3029843..3030627 Replicon chromosome
Accession NZ_CP071954
Organism Escherichia coli strain SB0258h1

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag J5X85_RS14680 Protein ID WP_000613626.1
Coordinates 3030133..3030627 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag J5X85_RS14675 Protein ID WP_001110447.1
Coordinates 3029843..3030136 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5X85_RS14665 (3024993) 3024993..3025952 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
J5X85_RS14670 (3026525) 3026525..3029710 + 3186 WP_001550951.1 ribonuclease E -
J5X85_RS14675 (3029843) 3029843..3030136 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
J5X85_RS14680 (3030133) 3030133..3030627 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
J5X85_RS14685 (3030722) 3030722..3031675 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
J5X85_RS14690 (3031687) 3031687..3033330 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
J5X85_RS14695 (3033396) 3033396..3034337 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
J5X85_RS14700 (3034337) 3034337..3035434 - 1098 WP_001441925.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T197172 WP_000613626.1 NZ_CP071954:3030133-3030627 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T197172 NZ_CP095765:c3996356-3995043 [Proteus mirabilis]
ATGGCGAGTCTTGATGTATATATGAATGAATACTATGTGGGTATCTTTACTAAAAAAAGCTCAGGTGCGCATTCATTCCA
ATATACTGAAGATTGGGTTAATCAACCAGGAAGTCGTCCCATCTCTCTATCGATGCCATTACAACTTCAAGAATATCAAG
GAGACTGTGTCTATAATTTCTTTGATAATCTATTACCTGATAATACTGAAATTAGAAATCGTATTGTGAGTCGCTATAAT
GCGAACTCAAATCAACCCTTTGACCTATTAGTGGCTATCGGTGCAGATACTGTTGGTGCATTAAGGTTACTTCCTAGTGG
CAGTACGCCTGCGAATATCAAAAAAATAGAATATCAAAAACTCAATGATATTGAGCTTGAAAAAATTCTACTAGGATATA
AATCCAATACTCCACTCGGTATGCTTAAAAAATATACTGATTTCAGAATTTCCATCGCTGGAGCACAAGAAAAAACGGCA
TTACTTTTAAAAGATAATGATTGGTGTGTGCCACATAATAACACACCAACTACCCATATTATTAAACTGCCAATTGGTGT
TATTGAAAGTCATTCTTATACAATGGATTTATCCAATAGTGTAGAAAATGAGTATCTTTGTACCTTAATTGCCAAAGAAT
TTGATCTTCCCGTCCCCCACTGCTCTATTATTAAAACAAAAAATGTTAAAGCGCTTGCTGTAGAACGTTTTGATAGAAAA
TATTCATCTGATAAATCGTGGATAATACGTTTACCTCAAGAAGATTTTTGCCAAATTTTAAATATACCTTCTGCGCTAAA
ATATGAAAATGAAGGAGGGCCTGGCATTATCGATATTATGAAATATTTACTTGGCTCATCAAATGCCGATCAAGATCGTT
TTCATTTTATGAAGGCACAAGTGCTATTTTGGCTTTTGGCTGCAACGGATGGACACGCAAAAAATTTTTCTGTTTTTATC
AACCAAGAAGGACGTTTCCATCTCACTCCTCTTTATGACATTATGTCCATCTATCCCAATTTCGGAGGACGAGGACTTAA
TCCACGAGATGCAAAACTTGCTATGGGATTAAAAGCAAGCCGAGGCAAAAAATACGCAATAGAACAAATTTTTCCTCGTC
ATTTCTACCAAACAGCTGAAGCTGTCGGTTTCGATCCTTCATTAATGAGCGATATTCTTCGTTATTTTTATCAAGAAATG
GACGATGTCATACAACGCGTAAAACAGCAATTACCCGAGGATTTCCCTTCTGAGATTAGCAATACAATATTAGATGGAAT
GAAAGCACGCGCTGTTAGATTAGCTAACATTTAG

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT197172 WP_001110447.1 NZ_CP071954:3029843-3030136 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT197172 NZ_CP095765:c3996614-3996357 [Proteus mirabilis]
ATGATCATAATTAATGCCTATCAACTCAGTGTGCATCTAAAAGATGTCCGATTAACAAAAAAACTGTCTCAAAGTAAAGT
CGCCCAAAAAGTAGGGATCCGACAAGATACCGTGTCCAATTTTGAAATTAATCCTAATTCCACCAAACTAGAAACCTTTT
TCAAACTGCTCTCCGCACTAAATTTAGAGATGGAAATTCATCCTAAAGATGCTAAAAATAATTTAGCACACAAAAGTGAA
TGGAAAGAGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References