Detailed information of TA system TA197171

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2652048..2652686
• Replicon: chromosome
• Accession: NZ_CP071954
• Organism: Escherichia coli strain SB0258h1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: J5X85_RS12780
• Protein ID: WP_000813795.1
• Coordinates: 2652510..2652686 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J5X85_RS12775
• Protein ID: WP_076797675.1
• Coordinates: 2652048..2652464 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5X85_RS12755 (2647200)	2647200..2648141	-	942	WP_001251315.1	ABC transporter permease	-
J5X85_RS12760 (2648142)	2648142..2649155	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
J5X85_RS12765 (2649173)	2649173..2650318	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
J5X85_RS12770 (2650563)	2650563..2651969	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
J5X85_RS12775 (2652048)	2652048..2652464	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J5X85_RS12780 (2652510)	2652510..2652686	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J5X85_RS12785 (2652908)	2652908..2653138	+	231	WP_023910283.1	YncJ family protein	-
J5X85_RS12790 (2653230)	2653230..2655191	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J5X85_RS12795 (2655264)	2655264..2655800	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
J5X85_RS12800 (2655892)	2655892..2657064	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T197171 WP_000813795.1 NZ_CP071954:c2652686-2652510 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T197171 NZ_CP095765:3706618-3707004 [Proteus mirabilis]
ATGGTTATTTATAAAACAAAGATGTTCAAAAATAGTTTCAAAAAAATAACGCTTACGAATGCAGAACTAATCGCTATCGC
TACCGAAGTCCTTGCAGGACAATTTGAGGCAGATCTAGGTGGTGGTGTTATCAAGAAACGTGCCTGTATCCAAGGAAAAG
GTAAAAGTAGCGGAATTAGAACAATTATATTTTATAAGCAAGGGAATAATTTATTCTTTGCTGATGGTTGGAAAAAATCG
AGTTTAAGCTCTAAAAAAACCAAAGAGATCACAGATGATGAACTTGAGTCCTATAAAGATCTAGCAAAAGATCTCTTCAA
TGCAAATCAAAATAAAATAGATAAAATGATTGCACTAGGCCTTTTAACAGAGGTTAGATATGACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT197171 WP_076797675.1 NZ_CP071954:c2652464-2652048 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT197171 NZ_CP095765:3706997-3707317 [Proteus mirabilis]
ATGACTGATAAAATGAAAGAAATGCTAAAATTTGCACAAGAAATGGAAGATGCAGGAGTCACTGATGGCAGTACAACTAA
ACGTATTGCCGCCAGAATAAAAGCGAAAGAATTACAGGCTAAGTATAAACATCCTACCCCTATGACAGGTGAAGATATAA
AAAGGATCCGTGAGCAAAATAATATCTCTCAATCTTCCCTTGCTTTGATATTGAATATGACTAAAGAAAGCGTTTCTAAG
TGGGAAAGAAACGAAATTAAGCCCAATGGTGCAGCACTACGACTTCTGCATTTAATAGATAAAAAAATATTATTCATCTA
G

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1