Detailed information of TA system TA197170

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2547458..2549046
• Replicon: chromosome
• Accession: NZ_CP071954
• Organism: Escherichia coli strain SB0258h1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: J5X85_RS12340
• Protein ID: WP_001551128.1
• Coordinates: 2547724..2549046 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J5X85_RS12335
• Protein ID: WP_001296726.1
• Coordinates: 2547458..2547724 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5X85_RS12335 (2547458)	2547458..2547724	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J5X85_RS12340 (2547724)	2547724..2549046	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
J5X85_RS12345 (2549246)	2549246..2550598	-	1353	WP_001551127.1	SIR2 family protein	-
J5X85_RS12350 (2550813)	2550813..2551845	-	1033	Protein_2448	ISNCY family transposase	-
J5X85_RS12355 (2552212)	2552212..2553111	-	900	WP_001551125.1	LysR family transcriptional regulator	-
J5X85_RS12360 (2553250)	2553250..2553558	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2550813..2551631	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T197170 WP_001551128.1 NZ_CP071954:2547724-2549046 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T197170 NZ_CP095765:3321373-3321696 [Proteus mirabilis]
ATGAGCTGGAAGATCGACTTTTACGATGGCGTTGAGGATCAGATCCTCGATATGCCACCCAAAATACAAGCTCGCATGAT
TAAGCTTTTAGAGCTGATGGAAAAGCATGGTGCAAACTTGGGGCCTCCTCATACTGAGTCTATGGGCGATGGGTTATTTG
AAATCCGCGCCAAAGCTCAAGAAGGTATTGGTAGAGGCTTATTTTGCTACTTAAAAGGGAAGCACATTTATGTGTTACAC
GCTTTTGTAAAAAAATCTCAAAAGACGCCCAAAAATGAGCTCAATCTTGCCAGAGATAGACAAAAAGAGGTTCAAAAATC
ATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT197170 WP_001296726.1 NZ_CP071954:2547458-2547724 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT197170 NZ_CP095765:3321693-3321971 [Proteus mirabilis]
ATGAGTCTGCAAAAACTGAAACAACGTGCTTTAGCCAATGCTGAAGTTAAGAATGAGTACGATAAGCTTGAGAGTGAGTT
CAGCTTTATTGACCAATTGCTTACCATGAGAACAAAGGCGGGGCTTACTCAAGAACAGGTTGCTGAAAGAATGCATACCC
AAAAAAGTAATGTCAGTCGTTTAGAAAAAGGTAATGCCAATCCAAGTTGGTCAACTCTTCTAAAGTATGCTCACGCATGT
GGCTTTGAACTAACACTCAAGCCACAAAAAATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A829LC11