Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1948685..1949516 Replicon chromosome
Accession NZ_CP071954
Organism Escherichia coli strain SB0258h1

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag J5X85_RS09225 Protein ID WP_000854814.1
Coordinates 1948685..1949059 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag J5X85_RS09230 Protein ID WP_001285585.1
Coordinates 1949148..1949516 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5X85_RS09185 (1944081) 1944081..1945247 + 1167 WP_000830156.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
J5X85_RS09190 (1945366) 1945366..1945839 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
J5X85_RS09195 (1946037) 1946037..1947095 + 1059 WP_001200891.1 FUSC family protein -
J5X85_RS09200 (1947267) 1947267..1947596 + 330 WP_000450409.1 DUF496 family protein -
J5X85_RS09205 (1947697) 1947697..1947831 - 135 WP_001297944.1 EutP/PduV family microcompartment system protein -
J5X85_RS09210 (1947951) 1947951..1948079 + 129 Protein_1826 transposase domain-containing protein -
J5X85_RS09215 (1948368) 1948368..1948448 - 81 Protein_1827 hypothetical protein -
J5X85_RS09220 (1948494) 1948494..1948688 - 195 WP_000988600.1 DUF5983 family protein -
J5X85_RS09225 (1948685) 1948685..1949059 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
J5X85_RS09230 (1949148) 1949148..1949516 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
J5X85_RS09235 (1949590) 1949590..1949811 - 222 WP_000692323.1 DUF987 domain-containing protein -
J5X85_RS09240 (1949874) 1949874..1950350 - 477 WP_001186726.1 RadC family protein -
J5X85_RS09245 (1950366) 1950366..1950845 - 480 WP_134259499.1 antirestriction protein -
J5X85_RS09250 (1950927) 1950927..1951745 - 819 WP_060626401.1 DUF932 domain-containing protein -
J5X85_RS09255 (1951845) 1951845..1952078 - 234 WP_001119729.1 DUF905 family protein -
J5X85_RS09260 (1952153) 1952153..1952608 - 456 WP_147712073.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T197161 WP_000854814.1 NZ_CP071954:c1949059-1948685 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T197161 NZ_CP095762:c5237445-5237062 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTACCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT197161 WP_001285585.1 NZ_CP071954:c1949516-1949148 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT197161 NZ_CP095762:c5237687-5237445 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References