Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumA-relB/upstrm_HI1419-dnstrm_HI1420
Location 265762..266349 Replicon chromosome
Accession NZ_CP071953
Organism Candidatus Kaistella beijingensis strain PM-38

Toxin (Protein)


Gene name PumA Uniprot ID -
Locus tag J4771_RS01175 Protein ID WP_124784682.1
Coordinates 265762..266061 (+) Length 100 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A3G8WVU0
Locus tag J4771_RS01180 Protein ID WP_055041555.1
Coordinates 266065..266349 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4771_RS01160 (J4771_01155) 262821..263363 + 543 WP_224135654.1 YcxB family protein -
J4771_RS01165 (J4771_01160) 263591..264229 + 639 WP_224135655.1 DUF4145 domain-containing protein -
J4771_RS01170 (J4771_01165) 264285..265289 - 1005 WP_224135656.1 IS110 family transposase -
J4771_RS01175 (J4771_01170) 265762..266061 + 300 WP_124784682.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J4771_RS01180 (J4771_01175) 266065..266349 + 285 WP_055041555.1 putative addiction module antidote protein Antitoxin
J4771_RS01185 (J4771_01180) 266571..267059 + 489 WP_224135657.1 DUF2007 domain-containing protein -
J4771_RS01190 (J4771_01185) 267283..267918 + 636 WP_224135658.1 hypothetical protein -
J4771_RS01195 (J4771_01190) 268143..268730 + 588 WP_224135659.1 hypothetical protein -
J4771_RS01200 (J4771_01195) 268986..269381 + 396 WP_224135660.1 hypothetical protein -
J4771_RS01205 (J4771_01200) 269391..270386 + 996 WP_224135661.1 HNH endonuclease signature motif containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 250121..285218 35097


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-97)

Antitoxin

Uncharacterized protein HI1420

(6-91)


Sequences

Toxin        


Download         Length: 100 a.a.        Molecular weight: 11798.76 Da        Isoelectric Point: 10.0546

>T197153 WP_124784682.1 NZ_CP071953:265762-266061 [Candidatus Kaistella beijingensis]
MYFIEKTNEFDKWFRKLKDIRAKAKILFRIQKLQNEEHFGDCKPVGNGISELRINFAKGYRIYFKETDGKIIILLIGGDK
SSQQKDIEKAKEIWEKIKE

Download         Length: 300 bp

>T197153 NZ_CP095762:1907990-1908092 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10136.69 Da        Isoelectric Point: 4.2958

>AT197153 WP_055041555.1 NZ_CP071953:266065-266349 [Candidatus Kaistella beijingensis]
MDISKFDIADYLDSNEMIAEYLNVVLEEGDDSEIVVAIGNIAKAIGMTKIAEETGMSRPSLYKALSEGAKPQFSTIMKVL
KAVGGQIRVNPITA

Download         Length: 285 bp

>AT197153 NZ_CP095762:c1908099-1907954 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A3G8WVU0

References