Detailed information of TA system TA197149

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 4127357..4128282
• Replicon: chromosome
• Accession: NZ_CP071947
• Organism: Pseudomonas aeruginosa strain 2020HL-00861

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J5V74_RS19380
• Protein ID: WP_033979787.1
• Coordinates: 4128100..4128282 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J5V74_RS31415
• Protein ID: WP_016561976.1
• Coordinates: 4127357..4128052 (-)
• Length: 232 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5V74_RS19340 (J5V74_19340)	4122615..4123466	-	852	WP_031753338.1	S24 family peptidase	-
J5V74_RS19345 (J5V74_19345)	4123565..4123783	+	219	WP_003451709.1	Cro/CI family transcriptional regulator	-
J5V74_RS19350 (J5V74_19350)	4123815..4124387	+	573	WP_022579921.1	hypothetical protein	-
J5V74_RS31410	4124390..4125274	+	885	WP_128663341.1	hypothetical protein	-
J5V74_RS19360 (J5V74_19360)	4125177..4125914	+	738	WP_243703379.1	replication protein P	-
J5V74_RS19365 (J5V74_19365)	4125914..4126357	+	444	WP_010793142.1	RusA family crossover junction endodeoxyribonuclease	-
J5V74_RS19370 (J5V74_19370)	4126386..4127255	+	870	WP_132468393.1	hypothetical protein	-
J5V74_RS31415	4127357..4128052	-	696	WP_016561976.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J5V74_RS19380 (J5V74_19380)	4128100..4128282	-	183	WP_033979787.1	type II toxin-antitoxin system HicA family toxin	Toxin
J5V74_RS19385 (J5V74_19385)	4128482..4128817	+	336	WP_003119044.1	phage holin, lambda family	-
J5V74_RS19390 (J5V74_19390)	4128814..4129431	+	618	WP_132468394.1	glycoside hydrolase family 19 protein	-
J5V74_RS31420 (J5V74_19395)	4129449..4129880	+	432	WP_171946230.1	hypothetical protein	-
J5V74_RS19400 (J5V74_19400)	4129977..4130714	+	738	WP_015648943.1	hypothetical protein	-
J5V74_RS19405 (J5V74_19405)	4130929..4131474	+	546	WP_031627904.1	terminase small subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4098394..4144039	45645

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-58)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6750.85 Da
• Isoelectric Point: 11.0775

>T197149 WP_033979787.1 NZ_CP071947:c4128282-4128100 [Pseudomonas aeruginosa]
MKFSEFRRWLKAQGVTFEAGKGGHFKITAPNGKQTTFADHGAKEMPEPTRKAIIKQLGFK

Nucleotide

• Length: 183 bp

>T197149 NZ_CP095762:324313-324681 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 232 a.a.
• Molecular weight: 25958.40 Da
• Isoelectric Point: 4.8091

>AT197149 WP_016561976.1 NZ_CP071947:c4128052-4127357 [Pseudomonas aeruginosa]
MYDYAIRFEQDDSASGVAVFCRDLPELNSFGDDREHAIREALDAIETTLSLYVDARKPIPEATPPEEGEHVVHLPSVTVA
KIALWNEMMKRGMRKSDLCKLLGIAQTQGDRLVDFLHTSKMEALESALAALDTKLTVRASYGNNVVKLRYMANTARGRVL
VIPGDADPKALPRQYEWLQEAPCRIEETVDDFEVGARYAMWNVEEAVNAIQSRGYFSFWLETTSGPRSATR

Nucleotide

• Length: 696 bp

>AT197149 NZ_CP095762:324095-324316 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA