197148

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /Acetyltransf_4(toxin)
Location 3336177..3337685 Replicon chromosome
Accession NZ_CP071947
Organism Pseudomonas aeruginosa strain 2020HL-00861

Toxin (Protein)


Gene name - Uniprot ID Q9HYX1
Locus tag J5V74_RS15415 Protein ID WP_003119682.1
Coordinates 3336177..3336764 (+) Length 196 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A5B3ABU1
Locus tag J5V74_RS15420 Protein ID WP_003119683.1
Coordinates 3336831..3337685 (+) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5V74_RS15410 (J5V74_15410) 3332432..3335911 - 3480 WP_003117605.1 PAS domain-containing hybrid sensor histidine kinase/response regulator -
J5V74_RS15415 (J5V74_15415) 3336177..3336764 + 588 WP_003119682.1 GNAT family protein Toxin
J5V74_RS15420 (J5V74_15420) 3336831..3337685 + 855 WP_003119683.1 AraC family transcriptional regulator Antitoxin
J5V74_RS15425 (J5V74_15425) 3337782..3339947 + 2166 WP_003115567.1 TonB-dependent receptor family protein -
J5V74_RS15430 (J5V74_15430) 3340122..3341996 + 1875 WP_003091605.1 MFS transporter -
J5V74_RS15435 (J5V74_15435) 3342056..3342265 - 210 WP_003115568.1 cold-shock protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(20-152)

Antitoxin

AraC-type arabinose-binding/dimerisation domain

(19-151)


Sequences

Toxin        


Download         Length: 196 a.a.        Molecular weight: 21901.23 Da        Isoelectric Point: 9.8676

>T197148 WP_003119682.1 NZ_CP071947:3336177-3336764 [Pseudomonas aeruginosa]
MSRRAPMFKPLPITLQRGALRLEPLVEADIPELVSLAEANREALQYMDGPTRPDWYRQSLAEQREGRALPLAVRLGVQLV
GTTRFAEFLPALPACEIGWTWLDQAQHGSGLNRMIKYLMLKHAFDNLRMVRVQLSTAASNLRAQGAIDKLGAQREGVLRN
HRRLAGGRLDDTFVYSITDHEWPQVKAALEASFTG

Download         Length: 588 bp

>T197148 NZ_CP095761:c4722360-4722103 [Klebsiella michiganensis]
ATGAATCTATCCCGTCAGGAACAACGTACCTTACACGTTCTCGCTAAAGGTGGCCGGATCGCGCACATCCGCGATGCATC
AGGCCGCGTCACCTCTGTTGAATGCTACAGCCGCGAAGGGCTCCTGCTCAGCGACTGCACGCTCGCCGTTTTCAAAAAAC
TCAAAACCAAAAAGCTGATTAAGTCCGTCAACGGACAGCCCTACCGCATCAATACCACCGGGCTGAATAACGTCCGCGCA
CAGCTGGACAATCGTTAA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31875.19 Da        Isoelectric Point: 7.5295

>AT197148 WP_003119683.1 NZ_CP071947:3336831-3337685 [Pseudomonas aeruginosa]
VPDETSGEHTHFWRAAELGGVELLHARYVEQRFAPHVHSSFALVIIEQGAQRFRHRGGEHLAPLGSMVLINPDEVHTGSK
AHDAGWLYRGYYPEMEQIDGILDELEIHHGGVPRFADSVIHDQPLARAFVHLHRLLAEHAGSLETQTRWRETVLALFRRH
ARLPEAPKAGQEPLAVARARELLAARLLDPPSLEELAAAVNLSPFHFARVFRRATGMPPHAWLRQRRLEQARALLKSGRA
PLEVALELGFADQSHLGRQFKQAYGVAPGEYRSACARSSLTIYE

Download         Length: 855 bp

>AT197148 NZ_CP095761:c4722092-4721541 [Klebsiella michiganensis]
ATGACAAACCATAGCTTCACTTTTCATGTCGCCTCTGAAGGCGATACCGATGACATTCTCGACGTCGAAACCCGGGCTTT
CGGCTACAGCAAAGAGGCCAGGCTGGTGGCCGATTTGCTCAACGATGAGAGCGCCTGCCCGACGTTATCGCTGCTGGCCA
GGCATAACGGAGAGGCGGTTGGGCATAGTTTATTCACCCGGGCGACGTTCAAAGGGGAGCCGGACTCGCCGATGATGCAC
ATCCTTGCTCCCCTGGCGGTGGTGCCGGAATACCAGGGCGTTGGCGTTGGCGGAGGGCTTATCCGGCACGGCATTGAGCA
GTTGAAAGCAATGGGAAGCCAGATGGTATTTGTTCTGGGCCACGCCGCTTACTACCCCAGGCACGGGTTTGAGCCATGCG
CGGGAGATAAAGGCTATCCGGCGCCTTATCCGATTCCCGAGGCGCATAAAGCCTGCTGGATGCTGCAGCCGATTTCGTCT
CAGCCGCTGGGACGAACGGGCCAGATACAGTGCGCCCGGGCGTTAATGAAACCCGAGCACTGGCGCGAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1YRE
PDB 8GXJ

Antitoxin

Source ID Structure
AlphaFold DB A0A5B3ABU1

References