Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 543719..544290 Replicon chromosome
Accession NZ_CP071931
Organism Enterococcus faecium strain VRE3355

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag J5F47_RS04155 Protein ID WP_002286801.1
Coordinates 543949..544290 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag J5F47_RS04150 Protein ID WP_002323011.1
Coordinates 543719..543949 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J5F47_RS04125 538962..540293 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
J5F47_RS04130 540315..540941 + 627 WP_002333420.1 cysteine hydrolase -
J5F47_RS04135 541124..541705 + 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
J5F47_RS04140 542314..542889 + 576 WP_002293673.1 SOS response-associated peptidase family protein -
J5F47_RS04145 543094..543432 - 339 WP_002286804.1 hypothetical protein -
J5F47_RS04150 543719..543949 + 231 WP_002323011.1 hypothetical protein Antitoxin
J5F47_RS04155 543949..544290 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J5F47_RS04160 545140..545325 + 186 WP_002304207.1 hypothetical protein -
J5F47_RS04165 545830..546024 + 195 WP_002295146.1 hypothetical protein -
J5F47_RS04170 546215..546464 + 250 Protein_519 transposase -
J5F47_RS04175 546708..547538 - 831 WP_002286789.1 manganese catalase family protein -
J5F47_RS04180 547746..549080 + 1335 WP_002333419.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T197142 WP_002286801.1 NZ_CP071931:543949-544290 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T197142 NZ_CP095760:488748-489098 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAAAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT197142 WP_002323011.1 NZ_CP071931:543719-543949 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT197142 NZ_CP095760:488461-488742 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCTGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References