Detailed information of TA system TA197139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB-HicA
• Location: 61634..62284
• Replicon: plasmid unnamed1
• Accession: NZ_CP071930
• Organism: Enterococcus faecium strain VRE3355

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J5F47_RS00705
• Protein ID: WP_002332741.1
• Coordinates: 62099..62284 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A829EWE8
• Locus tag: J5F47_RS00700
• Protein ID: WP_002305052.1
• Coordinates: 61634..62065 (-)
• Length: 144 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J5F47_RS00685	57057..57929	-	873	WP_020944763.1	ROK family protein	-
J5F47_RS00690	57942..59369	-	1428	WP_002332665.1	sucrose-6-phosphate hydrolase	-
J5F47_RS00695	59423..60697	-	1275	WP_002323715.1	MFS transporter	-
J5F47_RS00700	61634..62065	-	432	WP_002305052.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J5F47_RS00705	62099..62284	-	186	WP_002332741.1	type II toxin-antitoxin system HicA family toxin	Toxin
J5F47_RS00710	62377..62556	-	180	WP_002295613.1	hypothetical protein	-
J5F47_RS00715	62937..63644	-	708	WP_020944761.1	hypothetical protein	-
J5F47_RS00720	63637..64074	-	438	WP_020944760.1	hypothetical protein	-
J5F47_RS00725	64089..64313	-	225	WP_020944759.1	hypothetical protein	-
J5F47_RS00730	64313..64519	-	207	WP_002296239.1	hypothetical protein	-
J5F47_RS00735	64966..65226	-	261	WP_002296240.1	hypothetical protein	-
J5F47_RS00740	65592..66077	-	486	WP_014401510.1	single-stranded DNA-binding protein	-
J5F47_RS00745	66080..66979	-	900	WP_014401509.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..220241	220241

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (10-104)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6969.15 Da
• Isoelectric Point: 10.6196

>T197139 WP_002332741.1 NZ_CP071930:c62284-62099 [Enterococcus faecium]
MPLTGKEMLKLLKKNGWVERRQEGSHHHLYKDGVRITVPVHANQDLGRGLEQKILKDAGLK

Nucleotide

• Length: 186 bp

>T197139 NZ_CP095743:306505-306855 [Niallia sp. Man26]
TTGATTGTCAAACGTGGTGACGTTTATTTTGCAGACCTATCCCCAGTTGTTGGCTCAGAGCAAGGCGGTGTCCGTCCTGT
ACTTGTCATTCAAAACGACATCGGGAATCGGTTCAGTCCCACAGTCATTATTGCAGCAATTACGGCTCAAATTCAGAAGG
CTAAATTGCCGACTCATGTGGAAATTGACGCGAAGCGCTATGGTTTTGAGAGAGATTCAGTAATTCTTTTGGAACAAATT
CGCACGATAGATAAACAGAGGTTAACGGACAAAATTACCCATCTTGATGATGAAATGATGGAAAAAGTAGATGAAGCTCT
GCAAATAAGCTTGGGTCTCATTGAATTTTGA

Antitoxin

Protein

• Length: 144 a.a.
• Molecular weight: 15811.92 Da
• Isoelectric Point: 4.1638

>AT197139 WP_002305052.1 NZ_CP071930:c62065-61634 [Enterococcus faecium]
MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYGIAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDD
SFTTLIRVDVAKYLKDTELVKKTLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN

Nucleotide

• Length: 432 bp

>AT197139 NZ_CP095743:306219-306500 [Niallia sp. Man26]
GTGTCTGAATCCAGCGCAACGACAGAGATCATGATAAAATTACCAAAGCATTTATTATCTGAATTGGACGGCTTCGTAAA
GCAAGAAAATGTGAATCGTAGTGAATTTATTTATCAAGCAACGAAAATGTACCTTCGTGAACGGAAAAAGAGACATATCC
GGGAATCAATGAGAAGAGGCTACATGGAAATGGCAAAATTAAATTTATCCATTGCATCGGAAAGCTTTCTAGCAGAATAT
GAGGCAGAGCATACAGTAGAACGTCTAGTTAGCGGAGGATAA

Structures

Antitoxin

• AlphaFold DB: A0A829EWE8