Detailed information of TA system TA197131

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/COG3657-dnstrm_HI1420
• Location: 1269466..1269995
• Replicon: plasmid unnamed
• Accession: NZ_CP071915
• Organism: Ralstonia solanacearum strain SY1

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: J4H89_RS22255
• Protein ID: WP_237177642.1
• Coordinates: 1269768..1269995 (-)
• Length: 76 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J4H89_RS22250
• Protein ID: WP_016722339.1
• Coordinates: 1269466..1269771 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4H89_RS22220 (J4H89_22215)	1264643..1264927	-	285	WP_011003391.1	hypothetical protein	-
J4H89_RS22225 (J4H89_22220)	1265296..1265447	+	152	Protein_1017	IS5/IS1182 family transposase	-
J4H89_RS22235 (J4H89_22230)	1266491..1266799	+	309	WP_043897983.1	hypothetical protein	-
J4H89_RS26180	1267194..1267433	+	240	WP_089191147.1	hypothetical protein	-
J4H89_RS22240 (J4H89_22235)	1267483..1267770	-	288	WP_043898169.1	barstar family protein	-
J4H89_RS22245 (J4H89_22240)	1267940..1269253	-	1314	WP_064478230.1	MFS family transporter	-
J4H89_RS22250 (J4H89_22245)	1269466..1269771	-	306	WP_016722339.1	putative addiction module antidote protein	Antitoxin
J4H89_RS22255 (J4H89_22250)	1269768..1269995	-	228	WP_237177642.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4H89_RS22260 (J4H89_22255)	1270150..1271349	-	1200	WP_089190958.1	MFS transporter	-
J4H89_RS22265 (J4H89_22260)	1271477..1272298	-	822	WP_089190959.1	GntR family transcriptional regulator	-
J4H89_RS22270 (J4H89_22265)	1272476..1273027	-	552	WP_011003399.1	helix-turn-helix domain-containing protein	-
J4H89_RS22275 (J4H89_22270)	1273160..1273759	+	600	WP_064478235.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	acrB / htpB / adeG / cheY / cheB / cheW / motA / cyaB / flgC / flgG / fliM / fliG / fliI	1..2177065	2177065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-69)

Antitoxin

• Cro/C1-type helix-turn-helix domain (52-96)

Sequences

Toxin

Protein

• Length: 76 a.a.
• Molecular weight: 8567.83 Da
• Isoelectric Point: 6.7166

>T197131 WP_237177642.1 NZ_CP071915:c1269995-1269768 [Ralstonia solanacearum]
VRIDRLQMGHPGDMKAVRDGIRELRIDHGPGDRLYFVRHGVVLIVLLCGGDKSTQETDIRRAIDLSRRLDVDDME

Nucleotide

• Length: 228 bp

>T197131 NZ_CP095741:c2191228-2190869 [Luteibacter aegosomaticola]
GTGGCAACTGAAGCTGCAGGCCTACAGGAGAAGGCGGTCTCGACCGATGCCCCGGGAAAACGCATCCACGTAACTTGCTT
TGGTATCACCTTTTGGTACCATTCAATGCAAGCACGCCATCGCCGCACGCTCGAGCTCATCGCCGTCCACCCGGCCTCCG
GCAACGTCCGCTGGACCGATGTCGAAGCGCTATTCCGCCACCTCGGCGGAAAGATTCGCGAGGCGGAGGGTTCGCGAATT
GCCGTCGTTCTCTTCGGCGAGGTCAGGGTGTTCCACCGGCCCCACCCTCGCCCCGATACCGACAAAGGCGCGGTGGCCAG
CGTACGAAAATGGCTCAAAAGCAATGGAGTGATCGCATGA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10967.57 Da
• Isoelectric Point: 7.2780

>AT197131 WP_016722339.1 NZ_CP071915:c1269771-1269466 [Ralstonia solanacearum]
MTMAQRTIKTRPWDSAEHLRTEADIAAYLDACLEEAGDDPAFITHALGVVARSRGMTQLARETGMTREGLYKALSEGGNP
SFATVLKVIRALGIRLHAVPT

Nucleotide

• Length: 306 bp

>AT197131 NZ_CP095741:c2190872-2190534 [Luteibacter aegosomaticola]
ATGAACAACATCATGGTCATCGACGGGTACCGCGCGGTCGTCCAGTACGATCCGGAAATCGACATGTTCCGCGGCGAGTT
CCTCGAATTGAACGGCGGCGCCGATTTCTACGCCGCGGATGTCGCCGGCCTTCGCAAGGAGGGCCTTGTTTCGCTTCGTG
TCTTCCTTGAAGCCTGCCGCGAGGCCGGCATATCGCCCGTCCGCGTTTTCTCGGGCAAATTCCAGGCACGCGTATCGGCT
GAACTGCACCTGCGCTCCAGCGAAGCCGCGGCCGCGCGCGGCATCAGCCTCAACCAGTTTGTTCAGGGTGCGATGGAGCG
CGAATTGCTCGCTGTCTGA