Detailed information of TA system TA197130

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 3199652..3201238
• Replicon: chromosome
• Accession: NZ_CP071914
• Organism: Ralstonia solanacearum strain SY1

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9E8CN90
• Locus tag: J4H89_RS15080
• Protein ID: WP_064047702.1
• Coordinates: 3199652..3200989 (-)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A223GLT8
• Locus tag: J4H89_RS15085
• Protein ID: WP_028853251.1
• Coordinates: 3200993..3201238 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4H89_RS15055 (J4H89_15050)	3195307..3196047	+	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-
J4H89_RS15060 (J4H89_15055)	3196044..3196742	+	699	WP_058908491.1	protocatechuate 3,4-dioxygenase subunit alpha	-
J4H89_RS15065 (J4H89_15060)	3196890..3197486	+	597	WP_016727240.1	RNA 2',3'-cyclic phosphodiesterase	-
J4H89_RS15070 (J4H89_15065)	3197499..3198227	-	729	WP_011001393.1	DsbA family protein	-
J4H89_RS15075 (J4H89_15070)	3198453..3199655	-	1203	WP_071013776.1	MFS transporter	-
J4H89_RS15080 (J4H89_15075)	3199652..3200989	-	1338	WP_064047702.1	type II toxin-antitoxin system HipA family toxin	Toxin
J4H89_RS15085 (J4H89_15080)	3200993..3201238	-	246	WP_028853251.1	helix-turn-helix transcriptional regulator	Antitoxin
J4H89_RS15090 (J4H89_15085)	3201342..3202181	-	840	WP_071013779.1	oxaloacetate decarboxylase	-
J4H89_RS26005	3202205..3202327	-	123	Protein_2982	2-hydroxychromene-2-carboxylate isomerase	-
J4H89_RS15095 (J4H89_15090)	3202499..3203137	+	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
J4H89_RS15100 (J4H89_15095)	3203205..3203681	+	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
J4H89_RS15105 (J4H89_15100)	3203701..3204252	+	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
J4H89_RS15110 (J4H89_15105)	3204249..3205175	+	927	WP_071013782.1	muramoyltetrapeptide carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-63)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48768.81 Da
• Isoelectric Point: 6.7232

>T197130 WP_064047702.1 NZ_CP071914:c3200989-3199652 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T197130 NZ_CP095741:1147088-1147372 [Luteibacter aegosomaticola]
ATGAAAGAAAAATTTACGCGTTGGGATCCCGTCGATCATCTTCATACGGAGACCAATCAGGTTGCGTACCTAAATGCATG
CCTGGAGGAAGACCCCGGTGACGGGAGCCTCGTCCGTGCTGCATTAGGTGATATCGCCCGAGCCCGTGGCATGAGTTCGG
TGTCCCGGGCCGCCGGGATCTCTCGCGAGGGACTCTATCGAGCTTTGGCGACGGATGGGAATCCCGAATTCGCCACGGTA
ATGCGTGTGATGCGCGCGCTGGGTTTCCATCTTCGCGCGGCATGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8482.80 Da
• Isoelectric Point: 10.4693

>AT197130 WP_028853251.1 NZ_CP071914:c3201238-3200993 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Nucleotide

• Length: 246 bp

>AT197130 NZ_CP095741:1146855-1147088 [Luteibacter aegosomaticola]
ATGCTGCGCATTGATGCGCGACTTCGTCGTATGGCGCTCGGTCACTTTGGTGACGTCAAGCATGTCGGTGGGGGTGTTCG
AGAAGCGCGGATCGATTGCGGCCCCGGGTATCGCCTGTACTACATGCAGCGGCGCGGCGATTTGATCATTCTGCTATGTG
GGGGCGACAAGTCGACCCAGCAGCAGGACATCCGCAAGGCGCATGACATCGCTAACTCATGGGGCGAGAGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A223GLT8