Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 1157397..1158030 Replicon chromosome
Accession NZ_CP071914
Organism Ralstonia solanacearum strain SY1

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag J4H89_RS05405 Protein ID WP_016725172.1
Coordinates 1157397..1157810 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag J4H89_RS05410 Protein ID WP_011002949.1
Coordinates 1157797..1158030 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4H89_RS05385 (J4H89_05385) 1152776..1154512 + 1737 WP_019718916.1 ABC transporter substrate-binding protein -
J4H89_RS05390 (J4H89_05390) 1154674..1156170 + 1497 WP_071015012.1 glycerol kinase GlpK -
J4H89_RS05395 (J4H89_05395) 1156234..1156605 - 372 WP_071015009.1 hypothetical protein -
J4H89_RS05400 (J4H89_05400) 1156796..1157422 + 627 WP_071015005.1 DUF1415 family protein -
J4H89_RS05405 (J4H89_05405) 1157397..1157810 - 414 WP_016725172.1 PIN domain-containing protein Toxin
J4H89_RS05410 (J4H89_05410) 1157797..1158030 - 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
J4H89_RS05415 (J4H89_05415) 1158087..1159694 - 1608 WP_020832850.1 glucan biosynthesis protein -
J4H89_RS05420 (J4H89_05420) 1159907..1161139 + 1233 WP_250570813.1 AGE family epimerase/isomerase -
J4H89_RS05425 (J4H89_05425) 1161147..1162442 + 1296 WP_011002952.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T197129 WP_016725172.1 NZ_CP071914:c1157810-1157397 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T197129 NZ_CP095741:1146855-1147088 [Luteibacter aegosomaticola]
ATGCTGCGCATTGATGCGCGACTTCGTCGTATGGCGCTCGGTCACTTTGGTGACGTCAAGCATGTCGGTGGGGGTGTTCG
AGAAGCGCGGATCGATTGCGGCCCCGGGTATCGCCTGTACTACATGCAGCGGCGCGGCGATTTGATCATTCTGCTATGTG
GGGGCGACAAGTCGACCCAGCAGCAGGACATCCGCAAGGCGCATGACATCGCTAACTCATGGGGCGAGAGCTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT197129 WP_011002949.1 NZ_CP071914:c1158030-1157797 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT197129 NZ_CP095741:1147088-1147372 [Luteibacter aegosomaticola]
ATGAAAGAAAAATTTACGCGTTGGGATCCCGTCGATCATCTTCATACGGAGACCAATCAGGTTGCGTACCTAAATGCATG
CCTGGAGGAAGACCCCGGTGACGGGAGCCTCGTCCGTGCTGCATTAGGTGATATCGCCCGAGCCCGTGGCATGAGTTCGG
TGTCCCGGGCCGCCGGGATCTCTCGCGAGGGACTCTATCGAGCTTTGGCGACGGATGGGAATCCCGAATTCGCCACGGTA
ATGCGTGTGATGCGCGCGCTGGGTTTCCATCTTCGCGCGGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References