Detailed information of TA system TA197121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 1728376..1729121
• Replicon: chromosome
• Accession: NZ_CP071891
• Organism: Xanthomonas oryzae pv. oryzae strain T7133

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: J4Q40_RS08025
• Protein ID: WP_199285672.1
• Coordinates: 1728376..1728705 (+)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: J4Q40_RS08030
• Protein ID: WP_011407754.1
• Coordinates: 1728702..1729121 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4Q40_RS07995	1723507..1724178	+	672	WP_011407759.1	class I SAM-dependent methyltransferase	-
J4Q40_RS08005	1724480..1725271	-	792	WP_011407758.1	polysaccharide deacetylase family protein	-
J4Q40_RS08010	1725317..1726243	+	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
J4Q40_RS08015	1726575..1726910	+	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
J4Q40_RS08020	1726954..1728273	-	1320	WP_011407756.1	IS701 family transposase	-
J4Q40_RS08025	1728376..1728705	+	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
J4Q40_RS08030	1728702..1729121	+	420	WP_011407754.1	macro domain-containing protein	Antitoxin
J4Q40_RS08035	1729173..1730141	+	969	WP_011258802.1	IS5-like element ISXo1 family transposase	-
J4Q40_RS08040	1730276..1730932	+	657	WP_075246166.1	phosphate phosphatase	-
J4Q40_RS08045	1730970..1732205	-	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
J4Q40_RS08050	1732507..1732833	+	327	Protein_1562	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	1726954..1732205	5251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T197121 WP_199285672.1 NZ_CP071891:1728376-1728705 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T197121 NZ_CP095736:532381-532731 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT197121 WP_011407754.1 NZ_CP071891:1728702-1729121 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT197121 NZ_CP095736:532095-532376 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA