Detailed information of TA system TA197105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA(toxin)
• Location: 4151312..4153095
• Replicon: chromosome
• Accession: NZ_CP071876
• Organism: Enterobacter hormaechei strain GX4-8L

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: J4O68_RS20000
• Protein ID: WP_023314540.1
• Coordinates: 4151746..4153095 (-)
• Length: 450 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A837FFV9
• Locus tag: J4O68_RS19995
• Protein ID: WP_003859317.1
• Coordinates: 4151312..4151749 (-)
• Length: 146 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4O68_RS19975 (J4O68_19955)	4146332..4147354	+	1023	WP_045337656.1	HTH-type transcriptional regulator GalS	-
J4O68_RS19980 (J4O68_19960)	4147649..4148647	+	999	WP_003859322.1	galactose/glucose ABC transporter substrate-binding protein MglB	-
J4O68_RS19985 (J4O68_19965)	4148727..4150247	+	1521	WP_003859320.1	galactose/methyl galactoside ABC transporter ATP-binding protein MglA	-
J4O68_RS19990 (J4O68_19970)	4150263..4151273	+	1011	WP_003859318.1	galactose/methyl galactoside ABC transporter permease MglC	-
J4O68_RS19995 (J4O68_19975)	4151312..4151749	-	438	WP_003859317.1	helix-turn-helix transcriptional regulator	Antitoxin
J4O68_RS20000 (J4O68_19980)	4151746..4153095	-	1350	WP_023314540.1	HipA domain-containing protein	Toxin
J4O68_RS20005 (J4O68_19985)	4153230..4153940	-	711	WP_047054751.1	outer membrane permeability protein SanA	-
J4O68_RS20010 (J4O68_19990)	4154068..4154952	-	885	WP_003859312.1	cytidine deaminase	-
J4O68_RS20015 (J4O68_19995)	4155081..4155776	-	696	WP_235155270.1	CidB/LrgB family autolysis modulator	-
J4O68_RS20020 (J4O68_20000)	4155773..4156168	-	396	WP_003859308.1	CidA/LrgA family protein	-
J4O68_RS20025 (J4O68_20005)	4156337..4157272	+	936	WP_045343060.1	tRNA dihydrouridine(16) synthase DusC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-406)

Antitoxin

• Cro/C1-type helix-turn-helix domain (75-128)

Sequences

Toxin

Protein

• Length: 450 a.a.
• Molecular weight: 51137.78 Da
• Isoelectric Point: 4.6562

>T197105 WP_023314540.1 NZ_CP071876:c4153095-4151746 [Enterobacter hormaechei]
METLTVQAYLNETWTDIALIKYPGSEKNDWNTTQLDYLTEYAINFLDYDDFHAVSVNHPVSLFFDDHGQPGWLRFIDDII
PAGASRRYWINALDISELPVGQQNFLLLKFGTMSPVGNLRIKESVPEWNKFASTKTFTVADVIDRAADFLDYAQERGAAA
GGATGAGGEAPKLLLRCSETDAIWIDTWQNEPDNRDRYYLVKYPRGNRTEVDCNILRAEFHYYHELNAMGFSTIPVETMR
LEEGSHYPSLWLPRFDIVRNEQDQLVRLGMESVYSLLQKAPGTLLDHETTLRALINKITSSHMVQQQGYHFDIPGFVTEW
VRRDLLNIIFGNSDNHGRNTAFVKAENQIMLSPVYDFAPMKADPEGIPRTLKWSLSCESGGDYNFNTIAQTLAEWIPPAT
LLDALHETAVQLIDLPERLAARGVPEQIMEMPAMGFRYIPDKLSRWGLL

Nucleotide

• Length: 1350 bp

>T197105 NZ_CP095557:4038746-4038964 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 146 a.a.
• Molecular weight: 16185.64 Da
• Isoelectric Point: 10.5154

>AT197105 WP_003859317.1 NZ_CP071876:c4151749-4151312 [Enterobacter hormaechei]
MKTPNEAESELLQTLAQVRSVNKKRHHDEAEEPDKPSVVKRQRVTQGLTRISTLDRQAVLHAAIRDILLGKITQGEALKR
LRVEVLGLKQDEYAKLVSVSRKTLSDVENNRGNYSADVINKIFKPFGLQTGLVPVSKSLIASLFS

Nucleotide

• Length: 438 bp

>AT197105 NZ_CP095557:4038345-4038719 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A837FFV9