Detailed information of TA system TA197103

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2629067..2629683
• Replicon: chromosome
• Accession: NZ_CP071876
• Organism: Enterobacter hormaechei strain GX4-8L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J4O68_RS12725
• Protein ID: WP_080336832.1
• Coordinates: 2629312..2629683 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A6G4MTK3
• Locus tag: J4O68_RS12720
• Protein ID: WP_015569912.1
• Coordinates: 2629067..2629309 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4O68_RS12690 (J4O68_12670)	2624126..2624926	+	801	WP_126525051.1	DeoR/GlpR family DNA-binding transcription regulator	-
J4O68_RS12695 (J4O68_12675)	2625045..2625644	+	600	WP_003861947.1	glucose-1-phosphatase	-
J4O68_RS12700 (J4O68_12680)	2625638..2626519	+	882	WP_003861949.1	virulence factor BrkB family protein	-
J4O68_RS12705 (J4O68_12685)	2626516..2626953	+	438	WP_015569909.1	D-aminoacyl-tRNA deacylase	-
J4O68_RS12710 (J4O68_12690)	2626998..2627939	+	942	WP_015569910.1	fatty acid biosynthesis protein FabY	-
J4O68_RS12715 (J4O68_12695)	2627948..2628868	-	921	WP_235155183.1	alpha/beta hydrolase	-
J4O68_RS12720 (J4O68_12700)	2629067..2629309	+	243	WP_015569912.1	ribbon-helix-helix domain-containing protein	Antitoxin
J4O68_RS12725 (J4O68_12705)	2629312..2629683	+	372	WP_080336832.1	type II toxin-antitoxin system VapC family toxin	Toxin
J4O68_RS12730 (J4O68_12710)	2629723..2630652	-	930	WP_023323896.1	formate dehydrogenase accessory protein FdhE	-
J4O68_RS12735 (J4O68_12715)	2630649..2631284	-	636	WP_003861958.1	formate dehydrogenase cytochrome b556 subunit	-
J4O68_RS12740 (J4O68_12720)	2631281..2632183	-	903	WP_003861960.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13737.88 Da
• Isoelectric Point: 6.4882

>T197103 WP_080336832.1 NZ_CP071876:2629312-2629683 [Enterobacter hormaechei]
MEHMAVFDTNILIDLFNNRVEAADAIEHTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFAGIDEVLTPYQL

Nucleotide

• Length: 372 bp

>T197103 NZ_CP095557:c2701297-2699975 [Klebsiella pneumoniae]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
AAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCGCCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCACCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9440.72 Da
• Isoelectric Point: 4.4076

>AT197103 WP_015569912.1 NZ_CP071876:2629067-2629309 [Enterobacter hormaechei]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRNALGLWGDRKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT197103 NZ_CP095557:c2701563-2701297 [Klebsiella pneumoniae]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCACTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A6G4MTK3