Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1335811..1336431 Replicon chromosome
Accession NZ_CP071876
Organism Enterobacter hormaechei strain GX4-8L

Toxin (Protein)


Gene name Hha Uniprot ID A0A837FFM2
Locus tag J4O68_RS06520 Protein ID WP_015571250.1
Coordinates 1336213..1336431 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID F5RUW7
Locus tag J4O68_RS06515 Protein ID WP_006809850.1
Coordinates 1335811..1336185 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4O68_RS06505 (J4O68_06490) 1330939..1332132 + 1194 WP_022650498.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J4O68_RS06510 (J4O68_06495) 1332155..1335301 + 3147 WP_047054540.1 multidrug efflux RND transporter permease subunit AcrB -
J4O68_RS06515 (J4O68_06500) 1335811..1336185 + 375 WP_006809850.1 Hha toxicity modulator TomB Antitoxin
J4O68_RS06520 (J4O68_06505) 1336213..1336431 + 219 WP_015571250.1 HHA domain-containing protein Toxin
J4O68_RS06525 (J4O68_06510) 1336640..1337191 + 552 WP_022650496.1 maltose O-acetyltransferase -
J4O68_RS06530 (J4O68_06515) 1337308..1337775 + 468 WP_022650495.1 YlaC family protein -
J4O68_RS06535 (J4O68_06520) 1337747..1339207 - 1461 WP_022650494.1 PLP-dependent aminotransferase family protein -
J4O68_RS06540 (J4O68_06525) 1339309..1340019 + 711 WP_045337215.1 GNAT family protein -
J4O68_RS06545 (J4O68_06530) 1340016..1340156 - 141 WP_032608666.1 type B 50S ribosomal protein L36 -
J4O68_RS06550 (J4O68_06535) 1340159..1340419 - 261 WP_015571255.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8583.95 Da        Isoelectric Point: 8.9008

>T197100 WP_015571250.1 NZ_CP071876:1336213-1336431 [Enterobacter hormaechei]
MSDKPLTKVDYLMRLRRCQSIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFVR

Download         Length: 219 bp

>T197100 NZ_CP095557:336581-336949 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14497.28 Da        Isoelectric Point: 4.8989

>AT197100 WP_006809850.1 NZ_CP071876:1335811-1336185 [Enterobacter hormaechei]
MDEYSPKRHDIAQLKFLCESLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFTNVSRANPVSLSC

Download         Length: 375 bp

>AT197100 NZ_CP095557:336363-336584 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FFM2

Antitoxin

Source ID Structure
AlphaFold DB A0A837FGN8

References