Detailed information of TA system TA197097

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemK-mazE/MazF(toxin)
• Location: 1476491..1477129
• Replicon: chromosome
• Accession: NZ_CP071875
• Organism: Paenibacillus polymyxa strain SF05

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: H6CFY1
• Locus tag: J4I02_RS06955
• Protein ID: WP_007429335.1
• Coordinates: 1476779..1477129 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H6CFY0
• Locus tag: J4I02_RS06950
• Protein ID: WP_007429334.1
• Coordinates: 1476491..1476772 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4I02_RS06935	1473015..1473203	-	189	WP_274484462.1	hypothetical protein	-
J4I02_RS06940 (J4I02_06830)	1473467..1474744	+	1278	WP_274484463.1	outer membrane lipoprotein carrier protein LolA	-
J4I02_RS06945 (J4I02_06835)	1475003..1476199	+	1197	WP_274484464.1	alanine racemase	-
J4I02_RS06950 (J4I02_06840)	1476491..1476772	+	282	WP_007429334.1	ribbon-helix-helix protein, CopG family	Antitoxin
J4I02_RS06955 (J4I02_06845)	1476779..1477129	+	351	WP_007429335.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J4I02_RS06960 (J4I02_06850)	1477309..1479531	+	2223	WP_274485566.1	Tex family protein	-
J4I02_RS06965 (J4I02_06855)	1479637..1479771	-	135	WP_007429337.1	cortex morphogenetic protein CmpA	-
J4I02_RS06970 (J4I02_06860)	1479917..1480441	+	525	WP_274485567.1	SprT family protein	-
J4I02_RS06985 (J4I02_06875)	1480828..1481703	-	876	WP_274484465.1	Cof-type HAD-IIB family hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12822.77 Da
• Isoelectric Point: 5.6195

>T197097 WP_007429335.1 NZ_CP071875:1476779-1477129 [Paenibacillus polymyxa]
MIVKRGDVFFADLSPVVGSEQGGVRPVLIIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAATHGFDRDSVVLLEQI
RTIDKQRLTDKITHLDEETMRKVSDSLQISLGLIDF

Nucleotide

• Length: 351 bp

>T197097 NZ_CP095552:c4629886-4629311 [Gordonia hongkongensis]
GTGACTCCGCGGCTCGATCTGGTCGTCGGATGCAACGGCGCGGGCAAGTCGACGCTGGTCGAGCGTCATCTGCAACCCCT
GCTGCATGTGCCCTTCGTCAACGCCGATCTGATCGCCCGGCAGCGGTGGCCCGACTCCGCGGAAGCCCATTCCTACGACG
CCGCCCGGTTGGCGGCGGCGACCCGCGACACTCTCATCGAGCGCGGCACCTCGTTCATCGCCGAGACGGTCTTCTCGCAC
CCCTCGAAACTGGAGCTCATTGAACGTGCGCATACCGCCGGATACCGAGTCGCGCTGCACGTCGTGATGATCCCCGAGGC
TCTCGCGGTCCATCGCGTCCGATTGCGCGTGGACAGTGGCGGGCACGCGGTCCCGGAGGTCAAGATCCGCGAGCGGTTCC
GACGCCTGTGGCCGCTCGTCCGGACGGCCATCGCGCGGAGTGACCTGGCAACCGTGTACGCCAATGAGCGACCGACCACG
CGCATCGTCGCGCGGTACGTCGACGGTCTGGTCGCCGGCGTACCCGACTGGCCGGACTGGACGCCGGGTGCGCTGACGGA
TACGAAGGCACGATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10636.25 Da
• Isoelectric Point: 8.5257

>AT197097 WP_007429334.1 NZ_CP071875:1476491-1476772 [Paenibacillus polymyxa]
MANLQNTKRIMISLPDHLLQEVDGIVAMENSNRSEFIRQAMKLYVGERKKRYIREAMQRGYMEMAKINLTMASEAFHAEE
DANSTLGRTVSGV

Nucleotide

• Length: 282 bp

>AT197097 NZ_CP095552:c4630266-4629883 [Gordonia hongkongensis]
ATGAGCACCGGTACGGACAAGGTGACCCGGCTGTCTGCTGCATTGGTGACCGATGCGGCCCGGGAGGGCGCGCGAGAGCA
GCGGTCGGCGCGGCAGCAACTCGAACACTGGGCGCGTCTGGGGCGCAGCGTCTCGCAGCGCACCACCGCGTCGCGTCAGC
GGGTCGAGGCCGCGCTGGCGGGCGATCTGCCGGGAGAAGTGCTGACCGCAGAGGAAGCCGTCGTCTACGACGCCGAGATC
GACGCTCATCTCGAAGCGCGGCTCACGACCGACGACCACGTGGTTCGTCGTGCCTCGGCCGGGTTGTCCTCGGTGGTCGT
CGATGACGCCGGACGGCTCGTCGAACACCGCCCCGACGGGACGAGCGTCATCCTCGAGCAGTGA

Structures

Toxin

• AlphaFold DB: H6CFY1

Antitoxin

• AlphaFold DB: H6CFY0