Detailed information of TA system TA197092

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): zeta-pezA/zeta-couple_hipB
• Location: 1007858..1009101
• Replicon: chromosome
• Accession: NZ_CP071871
• Organism: Streptococcus pneumoniae strain BVJ1JL

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: JN057_RS05230
• Protein ID: WP_061638910.1
• Coordinates: 1008334..1009101 (+)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: B6ZCC4
• Locus tag: JN057_RS05225
• Protein ID: WP_000579612.1
• Coordinates: 1007858..1008334 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JN057_RS05200 (JN057_01065)	1002968..1003216	+	249	WP_001814254.1	hypothetical protein	-
JN057_RS05205 (JN057_01066)	1003387..1004106	+	720	Protein_1042	type A chloramphenicol O-acetyltransferase	-
JN057_RS05210 (JN057_01068)	1004902..1005825	-	924	WP_000417606.1	protein rep	-
JN057_RS05215 (JN057_01069)	1006029..1006859	+	831	Protein_1044	alpha-amylase family glycosyl hydrolase	-
JN057_RS05220 (JN057_01070)	1007127..1007582	+	456	WP_025173400.1	hypothetical protein	-
JN057_RS05225 (JN057_01071)	1007858..1008334	+	477	WP_000579612.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
JN057_RS05230 (JN057_01072)	1008334..1009101	+	768	WP_061638910.1	type II toxin-antitoxin system toxin PezT	Toxin
JN057_RS05235 (JN057_01073)	1009105..1010436	+	1332	WP_061370832.1	hypothetical protein	-
JN057_RS05240 (JN057_01074)	1010429..1011151	+	723	WP_061370831.1	hypothetical protein	-
JN057_RS05245 (JN057_01075)	1011123..1013783	+	2661	WP_061370834.1	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(M) / cat(pC194)	-	963639..1015409	51770
	-	flank	IS/Tn	cat(pC194)	-	1000895..1004037	3142

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 29241.42 Da
• Isoelectric Point: 8.3918

>T197092 WP_061638910.1 NZ_CP071871:1008334-1009101 [Streptococcus pneumoniae]
VEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKYFAGKMVEYLVTELSHFEYNLLIEGTLRTIDVPKKTAQLLKNKGYKVQLALIAAKPKLSYLSTLIRYEE
LYAINPNQARATPKEHHDFIVNHLVENTRQLEELAIFERIQIYQRDRSCVYDSGTDEGSAADVLQECLFGKLSKVEEEMM
KMGREQLRALKVKGE

Nucleotide

• Length: 768 bp

>T197092 NZ_CP095540:4536916-4537134 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGTCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCTGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18245.68 Da
• Isoelectric Point: 4.4188

>AT197092 WP_000579612.1 NZ_CP071871:1007858-1008334 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT197092 NZ_CP095540:4536516-4536890 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: B6ZCC4