Detailed information of TA system TA197087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): doc-mazE/Fic(toxin)
• Location: 765775..766504
• Replicon: chromosome
• Accession: NZ_CP071870
• Organism: Propioniciclava sp. MC1595

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: J4N02_RS03550
• Protein ID: WP_182818423.1
• Coordinates: 766142..766504 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: J4N02_RS03545
• Protein ID: WP_182818421.1
• Coordinates: 765775..766008 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4N02_RS03520 (J4N02_03520)	761525..761851	-	327	WP_188334165.1	transposase	-
J4N02_RS03525 (J4N02_03525)	761978..763264	-	1287	WP_188334164.1	ATP-binding protein	-
J4N02_RS03530 (J4N02_03530)	763530..763784	+	255	WP_188334163.1	hypothetical protein	-
J4N02_RS03535 (J4N02_03535)	764604..765326	+	723	WP_208091110.1	hypothetical protein	-
J4N02_RS03540 (J4N02_03540)	765457..765771	-	315	WP_182818420.1	type II toxin-antitoxin system PemK/MazF family toxin	-
J4N02_RS03545 (J4N02_03545)	765775..766008	-	234	WP_182818421.1	ribbon-helix-helix domain-containing protein	Antitoxin
J4N02_RS03550 (J4N02_03550)	766142..766504	-	363	WP_182818423.1	Fic family protein	Toxin
J4N02_RS03555 (J4N02_03555)	766501..766704	-	204	WP_182818424.1	CopG family transcriptional regulator	-
J4N02_RS17035 (J4N02_03565)	767350..767808	+	459	WP_260519454.1	NAD-glutamate dehydrogenase	-
J4N02_RS03570 (J4N02_03570)	767963..768841	-	879	WP_182818426.1	Gmad2 immunoglobulin-like domain-containing protein	-
J4N02_RS03575 (J4N02_03575)	768980..769885	+	906	WP_188334161.1	SDR family oxidoreductase	-
J4N02_RS03580 (J4N02_03580)	769979..771085	+	1107	WP_223202576.1	alcohol dehydrogenase catalytic domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	743613..802631	59018

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (6-80)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 12866.57 Da
• Isoelectric Point: 4.1358

>T197087 WP_182818423.1 NZ_CP071870:c766504-766142 [Propioniciclava sp. MC1595]
VIEYLTLEDVLALVADLGVGPIRDIGLLDSAVHRPHVTVFGRDAYPDLDAKAAVLLESVVRNHALVDGDKRLGWLAVVVF
YGLNDVILEAPDDDAYDLVIALASGTTDHPDAAAQLASWH

Nucleotide

• Length: 363 bp

>T197087 NZ_CP095540:c2897838-2897464 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTACTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTTGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8330.37 Da
• Isoelectric Point: 4.1265

>AT197087 WP_182818421.1 NZ_CP071870:c766008-765775 [Propioniciclava sp. MC1595]
MTVQIAIRLPDDMVAFLDKSVAAGNAPSRAALVAHAVEREMRRQVAEQDAAILREQGPSDDLDDLVAWSVAQATLED

Nucleotide

• Length: 234 bp

>AT197087 NZ_CP095540:c2898295-2897927 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCAGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA