Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 765457..766008 Replicon chromosome
Accession NZ_CP071870
Organism Propioniciclava sp. MC1595

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag J4N02_RS03540 Protein ID WP_182818420.1
Coordinates 765457..765771 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag J4N02_RS03545 Protein ID WP_182818421.1
Coordinates 765775..766008 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4N02_RS03510 (J4N02_03510) 760573..760721 - 149 Protein_699 transposase -
J4N02_RS03515 (J4N02_03515) 760736..761062 - 327 Protein_700 IS1634 family transposase -
J4N02_RS03520 (J4N02_03520) 761525..761851 - 327 WP_188334165.1 transposase -
J4N02_RS03525 (J4N02_03525) 761978..763264 - 1287 WP_188334164.1 ATP-binding protein -
J4N02_RS03530 (J4N02_03530) 763530..763784 + 255 WP_188334163.1 hypothetical protein -
J4N02_RS03535 (J4N02_03535) 764604..765326 + 723 WP_208091110.1 hypothetical protein -
J4N02_RS03540 (J4N02_03540) 765457..765771 - 315 WP_182818420.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
J4N02_RS03545 (J4N02_03545) 765775..766008 - 234 WP_182818421.1 ribbon-helix-helix domain-containing protein Antitoxin
J4N02_RS03550 (J4N02_03550) 766142..766504 - 363 WP_182818423.1 Fic family protein -
J4N02_RS03555 (J4N02_03555) 766501..766704 - 204 WP_182818424.1 CopG family transcriptional regulator -
J4N02_RS17035 (J4N02_03565) 767350..767808 + 459 WP_260519454.1 NAD-glutamate dehydrogenase -
J4N02_RS03570 (J4N02_03570) 767963..768841 - 879 WP_182818426.1 Gmad2 immunoglobulin-like domain-containing protein -
J4N02_RS03575 (J4N02_03575) 768980..769885 + 906 WP_188334161.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 743613..802631 59018


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-97)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11222.10 Da        Isoelectric Point: 5.8039

>T197086 WP_182818420.1 NZ_CP071870:c765771-765457 [Propioniciclava sp. MC1595]
VREICLVRLDKTRPAVVLTREAARAAMTKVTIAPITATIKGLSSEVPLGRTNGLDHDGVISLDNVVTVPSTLLGRTIWFL
TSAQETELARAIVLAYDLDIPLLD

Download         Length: 315 bp

>T197086 NZ_CP095540:2884802-2885056 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8330.37 Da        Isoelectric Point: 4.1265

>AT197086 WP_182818421.1 NZ_CP071870:c766008-765775 [Propioniciclava sp. MC1595]
MTVQIAIRLPDDMVAFLDKSVAAGNAPSRAALVAHAVEREMRRQVAEQDAAILREQGPSDDLDDLVAWSVAQATLED

Download         Length: 234 bp

>AT197086 NZ_CP095540:2884554-2884805 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References