Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 167131..167867 Replicon plasmid pGX1Z-1L
Accession NZ_CP071862
Organism Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag J4G41_RS24090 Protein ID WP_003026803.1
Coordinates 167131..167613 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag J4G41_RS24095 Protein ID WP_003026799.1
Coordinates 167601..167867 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G41_RS24060 (J4G41_24030) 162358..162993 + 636 WP_000677445.1 type 3 fimbria minor subunit MrkF -
J4G41_RS24065 (J4G41_24035) 163028..163180 - 153 Protein_179 EAL domain-containing protein -
J4G41_RS24070 (J4G41_24040) 163202..164170 - 969 WP_077256372.1 IS5 family transposase -
J4G41_RS24075 (J4G41_24045) 164284..164481 + 198 Protein_181 IS3 family transposase -
J4G41_RS24080 (J4G41_24050) 164709..165413 - 705 WP_031591821.1 toll/interleukin-1 receptor domain-containing protein -
J4G41_RS24085 (J4G41_24055) 165573..166919 - 1347 WP_077251107.1 ISNCY family transposase -
J4G41_RS24090 (J4G41_24060) 167131..167613 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
J4G41_RS24095 (J4G41_24065) 167601..167867 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
J4G41_RS24100 (J4G41_24070) 168112..168546 - 435 WP_001567367.1 cell envelope integrity protein TolA -
J4G41_RS24105 (J4G41_24075) 168593..169141 - 549 WP_001567366.1 thioredoxin fold domain-containing protein -
J4G41_RS24110 (J4G41_24080) 169593..169943 + 351 WP_004200891.1 hypothetical protein -
J4G41_RS24115 (J4G41_24085) 170170..170874 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J4G41_RS24120 (J4G41_24090) 170884..171351 + 468 Protein_190 DUF4158 domain-containing protein -
J4G41_RS24125 (J4G41_24095) 171443..172147 + 705 WP_136655513.1 IS6-like element IS26 family transposase -
J4G41_RS24130 (J4G41_24100) 172170..172295 - 126 Protein_192 IS1380 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid lnu(G) / ant(3'')-Ia / blaTEM-1B / qnrS1 / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / floR / tet(X4) mrkA / mrkB / mrkC / mrkD / mrkF 1..296468 296468
- inside IScluster/Tn qnrS1 - 163202..177610 14408


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T197081 WP_003026803.1 NZ_CP071862:c167613-167131 [Enterobacter cloacae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T197081 NZ_CP095540:c1600927-1600463 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT197081 WP_003026799.1 NZ_CP071862:c167867-167601 [Enterobacter cloacae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT197081 NZ_CP095540:c1601262-1600927 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTAACGCTGTACTGACCACTGAATCAAAGGTCACTATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCTGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAT
CCGCAAAAAACTCGTCCATTCGACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGACGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References