Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2103760..2104380 Replicon chromosome
Accession NZ_CP071861
Organism Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)


Gene name Hha Uniprot ID A0A5E1A1U8
Locus tag J4G41_RS10105 Protein ID WP_013095889.1
Coordinates 2103760..2103978 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID V3PBI9
Locus tag J4G41_RS10110 Protein ID WP_008499288.1
Coordinates 2104006..2104380 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G41_RS10075 (J4G41_10080) 2099769..2100029 + 261 WP_006176933.1 type B 50S ribosomal protein L31 -
J4G41_RS10080 (J4G41_10085) 2100032..2100172 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
J4G41_RS10085 (J4G41_10090) 2100169..2100879 - 711 WP_058682760.1 GNAT family protein -
J4G41_RS10090 (J4G41_10095) 2100981..2102444 + 1464 WP_058682759.1 PLP-dependent aminotransferase family protein -
J4G41_RS10095 (J4G41_10100) 2102413..2102883 - 471 WP_058682758.1 YlaC family protein -
J4G41_RS10100 (J4G41_10105) 2102999..2103550 - 552 WP_013095888.1 maltose O-acetyltransferase -
J4G41_RS10105 (J4G41_10110) 2103760..2103978 - 219 WP_013095889.1 HHA domain-containing protein Toxin
J4G41_RS10110 (J4G41_10115) 2104006..2104380 - 375 WP_008499288.1 Hha toxicity modulator TomB Antitoxin
J4G41_RS10115 (J4G41_10120) 2104893..2108039 - 3147 WP_013095890.1 multidrug efflux RND transporter permease subunit AcrB -
J4G41_RS10120 (J4G41_10125) 2108062..2109255 - 1194 WP_023620093.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8625.98 Da        Isoelectric Point: 8.9008

>T197070 WP_013095889.1 NZ_CP071861:c2103978-2103760 [Enterobacter cloacae]
MSDKPLTKTDYLMRLRRCQSIDTLERVIEKNKYELSDNELTVFYSAADHRLAELTMNKLYDKIPPSVWKFVR

Download         Length: 219 bp

>T197070 NZ_CP095538:4150748-4151005 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14481.28 Da        Isoelectric Point: 4.8886

>AT197070 WP_008499288.1 NZ_CP071861:c2104380-2104006 [Enterobacter cloacae]
MDEYSPKRHDIAQLKFLCESLYHDCLANLDESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINAQDLQKWRKSGNRLFRCFVNVSRANPVSLSC

Download         Length: 375 bp

>AT197070 NZ_CP095538:4151017-4151562 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1A1U8

Antitoxin

Source ID Structure
AlphaFold DB V3PBI9

References