Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 1651470..1652046 Replicon chromosome
Accession NZ_CP071861
Organism Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)


Gene name shpA Uniprot ID A0A5E1APA0
Locus tag J4G41_RS07965 Protein ID WP_023620650.1
Coordinates 1651470..1651757 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A5E1AP18
Locus tag J4G41_RS07970 Protein ID WP_023620649.1
Coordinates 1651744..1652046 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G41_RS07940 (J4G41_07950) 1646533..1647228 + 696 WP_038418621.1 winged helix-turn-helix domain-containing protein -
J4G41_RS07945 (J4G41_07955) 1647432..1647902 + 471 WP_013095432.1 MarR family transcriptional regulator -
J4G41_RS07950 (J4G41_07960) 1647899..1648966 + 1068 WP_154606842.1 HlyD family secretion protein -
J4G41_RS07955 (J4G41_07965) 1648956..1650032 + 1077 WP_013095434.1 DUF2955 domain-containing protein -
J4G41_RS07960 (J4G41_07970) 1650002..1651300 - 1299 WP_013095435.1 DUF445 domain-containing protein -
J4G41_RS07965 (J4G41_07975) 1651470..1651757 + 288 WP_023620650.1 BrnT family toxin Toxin
J4G41_RS07970 (J4G41_07980) 1651744..1652046 + 303 WP_023620649.1 BrnA antitoxin family protein Antitoxin
J4G41_RS07975 (J4G41_07985) 1652077..1652715 - 639 WP_163311637.1 LysE family translocator -
J4G41_RS07980 (J4G41_07990) 1652764..1653507 - 744 WP_058682779.1 AraC family transcriptional regulator -
J4G41_RS07985 (J4G41_07995) 1653645..1655015 + 1371 WP_058682778.1 NAD-dependent succinate-semialdehyde dehydrogenase -
J4G41_RS07990 (J4G41_08000) 1655057..1655378 - 322 Protein_1543 hypothetical protein -
J4G41_RS07995 (J4G41_08005) 1655378..1655923 - 546 WP_013095440.1 YfaZ family outer membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11246.60 Da        Isoelectric Point: 6.8813

>T197069 WP_023620650.1 NZ_CP071861:1651470-1651757 [Enterobacter cloacae]
MPTEYEWDSDKAKSNQQKHGIRFEDAVLVFDDPYHLSVQDRYENGEFRWQTIGLVQGVIVILVAHTVRFESGGEIIRIIS
ARKADRKERNRYEHR

Download         Length: 288 bp

>T197069 NZ_CP095538:4098556-4098897 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11414.05 Da        Isoelectric Point: 10.3694

>AT197069 WP_023620649.1 NZ_CP071861:1651744-1652046 [Enterobacter cloacae]
MSIVKHKRGSSPEVSARRDAELKALAEKPEAEIDYSDIPATDDKQWSGAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT197069 NZ_CP095538:c4098561-4098485 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1APA0

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AP18

References