Detailed information of TA system TA197067

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 1035442..1036058
• Replicon: chromosome
• Accession: NZ_CP071861
• Organism: Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J4G41_RS04990
• Protein ID: WP_080292722.1
• Coordinates: 1035687..1036058 (+)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B3JKE9
• Locus tag: J4G41_RS04985
• Protein ID: WP_020686881.1
• Coordinates: 1035442..1035684 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G41_RS04955 (J4G41_04970)	1030498..1031298	+	801	WP_020686879.1	DeoR/GlpR family DNA-binding transcription regulator	-
J4G41_RS04960 (J4G41_04975)	1031418..1032017	+	600	WP_058682050.1	glucose-1-phosphatase	-
J4G41_RS04965 (J4G41_04980)	1032011..1032892	+	882	WP_013099364.1	virulence factor BrkB family protein	-
J4G41_RS04970 (J4G41_04985)	1032889..1033326	+	438	WP_013099363.1	D-aminoacyl-tRNA deacylase	-
J4G41_RS04975 (J4G41_04990)	1033371..1034312	+	942	WP_013099362.1	fatty acid biosynthesis protein FabY	-
J4G41_RS04980 (J4G41_04995)	1034325..1035242	-	918	WP_058682051.1	alpha/beta hydrolase	-
J4G41_RS04985 (J4G41_05000)	1035442..1035684	+	243	WP_020686881.1	ribbon-helix-helix domain-containing protein	Antitoxin
J4G41_RS04990 (J4G41_05005)	1035687..1036058	+	372	WP_080292722.1	type II toxin-antitoxin system VapC family toxin	Toxin
J4G41_RS04995 (J4G41_05010)	1036098..1037027	-	930	WP_029882351.1	formate dehydrogenase accessory protein FdhE	-
J4G41_RS05000 (J4G41_05015)	1037024..1037659	-	636	WP_013099357.1	formate dehydrogenase cytochrome b556 subunit	-
J4G41_RS05005 (J4G41_05020)	1037656..1038558	-	903	WP_014833793.1	formate dehydrogenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-115)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13657.75 Da
• Isoelectric Point: 6.6407

>T197067 WP_080292722.1 NZ_CP071861:1035687-1036058 [Enterobacter cloacae]
MNNMAVFDTNILIDLFNNRVEAADAIDHAASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVLLR
EKHGMKLPDAIILATAQSRKCPLISRNTKDFSGIDEVVTPYQL

Nucleotide

• Length: 372 bp

>T197067 NZ_CP095538:c3709038-3708697 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9369.68 Da
• Isoelectric Point: 4.4076

>AT197067 WP_020686881.1 NZ_CP071861:1035442-1035684 [Enterobacter cloacae]
MSVLAELDMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIELYLDQQSSSVIRAALGLWGDKKEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT197067 NZ_CP095538:c3708687-3708388 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A8B3JKE9