Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PumAB/COG3657-dnstrm_HI1420
Location 677880..678476 Replicon chromosome
Accession NZ_CP071861
Organism Enterobacter cloacae strain GX1Z-1L

Toxin (Protein)


Gene name PumA Uniprot ID A0A0M7H115
Locus tag J4G41_RS03290 Protein ID WP_029882644.1
Coordinates 677880..678182 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name PumB Uniprot ID A0A0F1AUP1
Locus tag J4G41_RS03295 Protein ID WP_013095028.1
Coordinates 678189..678476 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G41_RS03275 (J4G41_03280) 674360..675709 + 1350 WP_163311542.1 lysine-sensitive aspartokinase 3 -
J4G41_RS03280 (J4G41_03285) 675802..676746 + 945 WP_013095031.1 ketopantoate/pantoate/pantothenate transporter PanS -
J4G41_RS03285 (J4G41_03290) 676814..677683 - 870 WP_013095030.1 23S rRNA pseudouridine(2604) synthase RluF -
J4G41_RS03290 (J4G41_03295) 677880..678182 + 303 WP_029882644.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J4G41_RS03295 (J4G41_03300) 678189..678476 + 288 WP_013095028.1 putative addiction module antidote protein Antitoxin
J4G41_RS03300 (J4G41_03305) 678473..680104 - 1632 WP_013095027.1 Na/Pi cotransporter family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Addiction module killer protein, predicted

(1-93)

Antitoxin

Uncharacterized protein HI1420

(3-90)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11457.30 Da        Isoelectric Point: 9.7662

>T197065 WP_029882644.1 NZ_CP071861:677880-678182 [Enterobacter cloacae]
MKEIVQTESFRRWEQNLKDRRARTIIASRLFRLANGLAGDIKPVGEGICELRIHFGPGYRIYFKDQGNCIIVLLCGGDKS
SQARDILMAKMLSNVSQWQE

Download         Length: 303 bp

>T197065 NZ_CP095538:3030756-3031142 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10070.62 Da        Isoelectric Point: 5.7807

>AT197065 WP_013095028.1 NZ_CP071861:678189-678476 [Enterobacter cloacae]
MHKLTPYDPAIALVDDEEIAVFMADALETGDSAYIAKALGVIARAKGMSTISQQTGLSREQLYRSFSDKGNPTLKTTLAV
MKALGLGLTIKPAGD

Download         Length: 288 bp

>AT197065 NZ_CP095538:3031132-3031461 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M7H115

Antitoxin

Source ID Structure
AlphaFold DB A0A0F1AUP1

References