Detailed information of TA system TA197022

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PanAT/DUF4065(antitoxin)
• Location: 167671..169358
• Replicon: chromosome
• Accession: NZ_CP071840
• Organism: Vibrio sp. SCSIO 43097

Toxin (Protein)

• Gene name: panT
• Uniprot ID: -
• Locus tag: J4N40_RS00845
• Protein ID: WP_025593654.1
• Coordinates: 168135..169358 (+)
• Length: 408 a.a.

Antitoxin (Protein)

• Gene name: panA
• Uniprot ID: -
• Locus tag: J4N40_RS00840
• Protein ID: WP_005378671.1
• Coordinates: 167671..168138 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4N40_RS00810 (J4N40_00810)	162811..163020	-	210	WP_025593645.1	AlpA family transcriptional regulator	-
J4N40_RS00815 (J4N40_00815)	163098..164087	+	990	WP_025593646.1	50S ribosome-binding GTPase	-
J4N40_RS00820 (J4N40_00820)	164197..164643	+	447	WP_025593648.1	hypothetical protein	-
J4N40_RS00825 (J4N40_00825)	164683..165327	-	645	WP_025593649.1	inovirus Gp2 family protein	-
J4N40_RS00830 (J4N40_00830)	165510..166526	-	1017	WP_229644735.1	hypothetical protein	-
J4N40_RS00835 (J4N40_00835)	166671..167342	+	672	WP_025593651.1	hypothetical protein	-
J4N40_RS00840 (J4N40_00840)	167671..168138	+	468	WP_005378671.1	DUF4065 domain-containing protein	Antitoxin
J4N40_RS00845 (J4N40_00845)	168135..169358	+	1224	WP_025593654.1	RelA/SpoT domain-containing protein	Toxin
J4N40_RS00850 (J4N40_00850)	169550..170659	-	1110	WP_064376997.1	tRNA (uridine(54)-C5)-methyltransferase TrmA	-
J4N40_RS00855 (J4N40_00855)	171100..172935	+	1836	WP_054579652.1	TonB-dependent vitamin B12 receptor	-
J4N40_RS00860 (J4N40_00860)	172995..173687	+	693	WP_005378675.1	ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	149710..169358	19648

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RelA/SpoT (111-228)

Antitoxin

• Antitoxin SocA-like, Panacea domain (32-123)

Sequences

Toxin

Protein

• Length: 408 a.a.
• Molecular weight: 45849.63 Da
• Isoelectric Point: 5.3830

>T197022 WP_025593654.1 NZ_CP071840:168135-169358 [Vibrio sp. SCSIO 43097]
VSNKENSVVSFYIDDTPTAEQASKTFQDDYELDFQDFIERTVNESAAEDFSYSNNGVKKAGRRLRKREGDLKSATATIQQ
FRAAHEKPLNTIAYLVGRCCRELGIDVKPVTRLKRLETIVDKLQRKSLDGETANATCVTNMNDIGGCRAIFPDLISLEKV
KSQLQLTVEKVGRVRIKDIDDYITSPKPNDCGYRSLHVIYRYDHASGKSFNIEAQLRTRLQHLWATTVEIVDILEGTKIK
THSHSSNEGKSSLQVQWEELLSIMSQCIADAEGAIELSDIDKADFSKRLVVLDREINALNRLSSFKILSEKVNSYCDSSI
EHVLLVINETSRELIFSEEFENYSQAISVYNAAEKVTRSYSRATSRLNTLLISTKNMGQLSEAYPNYLGDCASFITLLED
TMLSGES

Nucleotide

• Length: 1224 bp

>T197022 NZ_CP095529:2345557-2345733 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGTGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17516.97 Da
• Isoelectric Point: 7.9061

>AT197022 WP_005378671.1 NZ_CP071840:167671-168138 [Vibrio sp. SCSIO 43097]
MKAYHTYSAVDVAWSLLKHAKEQGKCFSNLQLQKLTYVCHGLSLAHFQRPLVVDDVFAWKFGPVVPSVYFRFKSYGSSVI
TEQCDVALDQESESIVKDVVSKLGHLTGPQLVDLTHREGSPWHQVWDGTHQKVIPDHLIQAHYTQIKQSGRTASL

Nucleotide

• Length: 468 bp

>AT197022 NZ_CP095529:2345779-2346195 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA