Detailed information of TA system TA197000

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 102919..103562
• Replicon: plasmid p90CM2-172k
• Accession: NZ_CP071821
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: IM758_RS27590
• Protein ID: WP_000754566.1
• Coordinates: 102919..103335 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: IM758_RS27595
• Protein ID: WP_001261276.1
• Coordinates: 103332..103562 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM758_RS27560 (IM758_27560)	98427..98633	+	207	WP_105910591.1	hypothetical protein	-
IM758_RS27565 (IM758_27565)	98997..100001	-	1005	WP_021546935.1	IS110-like element IS4321 family transposase	-
IM758_RS27570 (IM758_27570)	100173..100730	+	558	Protein_119	3'-5' exonuclease	-
IM758_RS27575 (IM758_27575)	100783..101178	+	396	WP_105910593.1	hypothetical protein	-
IM758_RS27580 (IM758_27580)	101175..101786	+	612	WP_105910594.1	DUF2913 family protein	-
IM758_RS27585 (IM758_27585)	101783..102712	-	930	WP_105910612.1	Rpn family recombination-promoting nuclease/putative transposase	-
IM758_RS27590 (IM758_27590)	102919..103335	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
IM758_RS27595 (IM758_27595)	103332..103562	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
IM758_RS27600 (IM758_27600)	104153..105097	+	945	WP_215116491.1	hypothetical protein	-
IM758_RS27605 (IM758_27605)	105134..105526	+	393	WP_023317792.1	hypothetical protein	-
IM758_RS27610 (IM758_27610)	105584..106105	+	522	WP_215116500.1	hypothetical protein	-
IM758_RS27615 (IM758_27615)	106151..106936	+	786	WP_215116492.1	site-specific integrase	-
IM758_RS27620 (IM758_27620)	107056..107271	-	216	WP_129586912.1	hypothetical protein	-
IM758_RS27625 (IM758_27625)	107318..108493	-	1176	Protein_130	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA	1..172589	172589
	-	flank	IS/Tn	-	-	98997..100001	1004

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T197000 WP_000754566.1 NZ_CP071821:c103335-102919 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T197000 NZ_CP095526:3423458-3423799 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT197000 WP_001261276.1 NZ_CP071821:c103562-103332 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT197000 NZ_CP095526:c3423463-3423387 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023