Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 89405..90048 Replicon plasmid p90CM2-172k
Accession NZ_CP071821
Organism Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag IM758_RS27505 Protein ID WP_012540249.1
Coordinates 89405..89821 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag IM758_RS27510 Protein ID WP_001261276.1
Coordinates 89818..90048 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM758_RS27490 (IM758_27490) 84453..86561 - 2109 WP_064147994.1 peptidase domain-containing ABC transporter -
IM758_RS27495 (IM758_27495) 86551..87828 - 1278 WP_064147993.1 HlyD family secretion protein -
IM758_RS27500 (IM758_27500) 88486..89364 + 879 WP_064147992.1 restriction endonuclease -
IM758_RS27505 (IM758_27505) 89405..89821 - 417 WP_012540249.1 type II toxin-antitoxin system VapC family toxin Toxin
IM758_RS27510 (IM758_27510) 89818..90048 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
IM758_RS27515 (IM758_27515) 90180..90521 + 342 Protein_108 IS3 family transposase -
IM758_RS27520 (IM758_27520) 90527..90781 - 255 WP_094307695.1 hypothetical protein -
IM758_RS27525 (IM758_27525) 91204..91992 - 789 Protein_110 sulfatase-like hydrolase/transferase -
IM758_RS27530 (IM758_27530) 92281..92745 - 465 WP_094307693.1 N-acetylneuraminate anomerase -
IM758_RS27535 (IM758_27535) 92742..93617 - 876 WP_094307692.1 N-acetylmannosamine kinase -
IM758_RS27540 (IM758_27540) 93614..94303 - 690 WP_094307691.1 N-acetylmannosamine-6-phosphate 2-epimerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - iucA / iucB / iucC / iucD / iutA 1..172589 172589
- flank IS/Tn - - 90135..90521 386


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15050.53 Da        Isoelectric Point: 8.5403

>T196999 WP_012540249.1 NZ_CP071821:c89821-89405 [Klebsiella pneumoniae subsp. pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Download         Length: 417 bp

>T196999 NZ_CP095526:3331700-3331852 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT196999 WP_001261276.1 NZ_CP071821:c90048-89818 [Klebsiella pneumoniae subsp. pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT196999 NZ_CP095526:c3331651-3331594 [Escherichia coli]
GTTCAGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References