Detailed information of TA system TA196998

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 73853..74589
• Replicon: plasmid p90CM2-172k
• Accession: NZ_CP071821
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: IM758_RS27420
• Protein ID: WP_077269838.1
• Coordinates: 73853..74335 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: IM758_RS27425
• Protein ID: WP_003026799.1
• Coordinates: 74323..74589 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM758_RS29110	69105..69239	-	135	Protein_82	IS110 family transposase	-
IM758_RS27390 (IM758_27390)	69434..69982	+	549	WP_143934746.1	thioredoxin fold domain-containing protein	-
IM758_RS29115	70460..70660	-	201	WP_236052301.1	hypothetical protein	-
IM758_RS27405 (IM758_27405)	71077..71691	+	615	WP_045420631.1	DJ-1/PfpI family protein	-
IM758_RS27410 (IM758_27410)	71703..72221	+	519	WP_047038610.1	GrpB family protein	-
IM758_RS27415 (IM758_27415)	72265..73674	-	1410	WP_038991643.1	ISNCY family transposase	-
IM758_RS27420 (IM758_27420)	73853..74335	-	483	WP_077269838.1	GNAT family N-acetyltransferase	Toxin
IM758_RS27425 (IM758_27425)	74323..74589	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
IM758_RS27430 (IM758_27430)	75251..76168	+	918	WP_069985721.1	bifunctional helix-turn-helix transcriptional regulator/GNAT family N-acetyltransferase	-
IM758_RS27435 (IM758_27435)	76878..77081	-	204	WP_004213596.1	HHA domain-containing protein	-
IM758_RS27440 (IM758_27440)	77142..77636	-	495	WP_004213594.1	hypothetical protein	-
IM758_RS27445 (IM758_27445)	77667..78233	-	567	WP_009654312.1	hypothetical protein	-
IM758_RS27450 (IM758_27450)	78230..78493	-	264	WP_009310051.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	iucA / iucB / iucC / iucD / iutA	1..172589	172589
	-	flank	IS/Tn	-	-	72265..73674	1409

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17378.94 Da
• Isoelectric Point: 9.6047

>T196998 WP_077269838.1 NZ_CP071821:c74335-73853 [Klebsiella pneumoniae subsp. pneumoniae]
VGRITAPEPLSSSHQRAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T196998 NZ_CP095526:c3032739-3032398 [Escherichia coli]
ATGTGGGACGTCGAGACAACGGAGACTTTCGACAACTGGTTTGATGCCCAGACTGTTGCGCTGAAAGAAGATTTACTTGC
TGCAATGCTGATTCTTGCTGAGTATGGCCCACAGTTGGGCAGACCCTTTGCGGATACGGTTAATGATTCACAGTTTTCAA
ATATGAAAGAATTACGCGTCCAACATCAGGGAAACCCGGTACGTGCTTTTTTTGCTTTCGATCCTGCGCGCCGTGGCATT
GTATTATGTGCGGGTGATAAAACGGGGTTAAATGAGAAAAAGTTTTATCGCGATATGATCAAGCTCGCTGATTCTGAATA
CCGTAAGCATCTGAAAAAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT196998 WP_003026799.1 NZ_CP071821:c74589-74323 [Klebsiella pneumoniae subsp. pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT196998 NZ_CP095526:c3032388-3032089 [Escherichia coli]
ATGGCTACTCTAAAAGAACTGATGGCAAAGCAAAGCCCTGAAAGCCAGGTGCGTATTGCGGCAAAAGTTGAGGAAATGCG
CCAGACCATCGCCTTGCATCAGCTGAGAGAAGAACTCAATATTTCGCAAACGGAAATGGCGGCAGCGATGGGCATTAAGC
AACCGACTCTTGCAAAAATGGAGCAGGCAGATAACGATCCTCGGTTGTCTACGCTAAAACGCTATGTTGCGGCTTTAGGT
GGGGAATTGAGTATCGACGTTAAACTGCCGAATGGTAAGAGAATTGCATTTCAGATTTAA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66