Detailed information of TA system TA196997

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 35743..36653
• Replicon: plasmid p90CM2-MCR-253k
• Accession: NZ_CP071820
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: IM758_RS25945
• Protein ID: WP_004144067.1
• Coordinates: 36183..36653 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: IM758_RS25940
• Protein ID: WP_032413443.1
• Coordinates: 35743..36186 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM758_RS25925 (IM758_25920)	32376..33863	+	1488	WP_215116437.1	group II intron reverse transcriptase/maturase	-
IM758_RS25930 (IM758_25925)	33949..34371	+	423	WP_004181893.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
IM758_RS25935 (IM758_25930)	34371..35642	+	1272	WP_004181894.1	translesion error-prone DNA polymerase V subunit UmuC	-
IM758_RS25940 (IM758_25935)	35743..36186	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
IM758_RS25945 (IM758_25940)	36183..36653	+	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
IM758_RS25950 (IM758_25945)	37106..37294	-	189	WP_065813301.1	hypothetical protein	-
IM758_RS25955 (IM758_25950)	37908..38168	-	261	WP_004026352.1	hypothetical protein	-
IM758_RS25960 (IM758_25955)	38806..39057	+	252	WP_139988952.1	DUF1158 domain-containing protein	-
IM758_RS25965 (IM758_25960)	39075..39416	-	342	WP_004202492.1	RamA family antibiotic efflux transcriptional regulator	-
IM758_RS25970 (IM758_25965)	39447..39794	-	348	Protein_51	MBL fold metallo-hydrolase	-
IM758_RS25975 (IM758_25970)	40003..41265	-	1263	WP_000608644.1	IS1380-like element ISEcp1 family transposase	-
IM758_RS29070	41505..41600	+	96	Protein_53	Hok/Gef family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / mcr-2.2 / mcr-3.11 / qnrS1 / mph(A) / aph(3')-IIa / blaCTX-M-63	-	1..253649	253649

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T196997 WP_004144067.1 NZ_CP071820:36183-36653 [Klebsiella pneumoniae subsp. pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T196997 NZ_CP095526:2806821-2807039 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT196997 WP_032413443.1 NZ_CP071820:35743-36186 [Klebsiella pneumoniae subsp. pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT196997 NZ_CP095526:2806421-2806795 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A7H4PRG4