Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5207904..5208529 Replicon chromosome
Accession NZ_CP071819
Organism Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag IM758_RS25350 Protein ID WP_002882817.1
Coordinates 5207904..5208287 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag IM758_RS25355 Protein ID WP_004150355.1
Coordinates 5208287..5208529 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM758_RS25335 (5205270) 5205270..5206172 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
IM758_RS25340 (5206169) 5206169..5206804 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
IM758_RS25345 (5206801) 5206801..5207730 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
IM758_RS25350 (5207904) 5207904..5208287 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
IM758_RS25355 (5208287) 5208287..5208529 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
IM758_RS25360 (5208734) 5208734..5209651 + 918 WP_023302328.1 alpha/beta hydrolase -
IM758_RS25365 (5209665) 5209665..5210606 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
IM758_RS25370 (5210651) 5210651..5211088 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
IM758_RS25375 (5211085) 5211085..5211945 - 861 WP_004146232.1 virulence factor BrkB family protein -
IM758_RS25380 (5211939) 5211939..5212538 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T196996 WP_002882817.1 NZ_CP071819:c5208287-5207904 [Klebsiella pneumoniae subsp. pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T196996 NZ_CP095526:2315106-2315492 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT196996 WP_004150355.1 NZ_CP071819:c5208529-5208287 [Klebsiella pneumoniae subsp. pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT196996 NZ_CP095526:2315482-2315811 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGAAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGACCGGAAGTTTT
TGCAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References