Detailed information of TA system TA196993

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/DUF1778(antitoxin)
• Location: 4624912..4625722
• Replicon: chromosome
• Accession: NZ_CP071819
• Organism: Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: A0A332NV98
• Locus tag: IM758_RS22615
• Protein ID: WP_014908042.1
• Coordinates: 4624912..4625445 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: -
• Locus tag: IM758_RS22620
• Protein ID: WP_023343051.1
• Coordinates: 4625456..4625722 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IM758_RS22610 (4623743)	4623743..4624864	+	1122	WP_012737335.1	cupin domain-containing protein	-
IM758_RS22615 (4624912)	4624912..4625445	-	534	WP_014908042.1	type II toxin-antitoxin system toxin KacT	Toxin
IM758_RS22620 (4625456)	4625456..4625722	-	267	WP_023343051.1	type II toxin-antitoxin system antitoxin KacA	Antitoxin
IM758_RS22625 (4625825)	4625825..4627258	-	1434	WP_023343050.1	Cu(+)/Ag(+) sensor histidine kinase	-
IM758_RS22630 (4627248)	4627248..4627931	-	684	WP_002887273.1	copper response regulator transcription factor CusR	-
IM758_RS22635 (4628104)	4628104..4629489	+	1386	WP_024623207.1	efflux transporter outer membrane subunit	-
IM758_RS22640 (4629507)	4629507..4629851	+	345	WP_004192220.1	cation efflux system protein CusF	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (87-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 19795.68 Da
• Isoelectric Point: 5.2614

>T196993 WP_014908042.1 NZ_CP071819:c4625445-4624912 [Klebsiella pneumoniae subsp. pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGLGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Nucleotide

• Length: 534 bp

>T196993 NZ_CP095526:c1615303-1615127 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10021.46 Da
• Isoelectric Point: 10.5807

>AT196993 WP_023343051.1 NZ_CP071819:c4625722-4625456 [Klebsiella pneumoniae subsp. pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRRVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Nucleotide

• Length: 267 bp

>AT196993 NZ_CP095526:c1615081-1614665 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A332NV98