Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4018414..4019033 Replicon chromosome
Accession NZ_CP071819
Organism Klebsiella pneumoniae subsp. pneumoniae strain 90CM2

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag IM758_RS19695 Protein ID WP_002892050.1
Coordinates 4018815..4019033 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag IM758_RS19690 Protein ID WP_002892066.1
Coordinates 4018414..4018788 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IM758_RS19680 (4013566) 4013566..4014759 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
IM758_RS19685 (4014782) 4014782..4017928 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
IM758_RS19690 (4018414) 4018414..4018788 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
IM758_RS19695 (4018815) 4018815..4019033 + 219 WP_002892050.1 HHA domain-containing protein Toxin
IM758_RS19700 (4019192) 4019192..4019758 + 567 WP_002892030.1 maltose O-acetyltransferase -
IM758_RS19705 (4019730) 4019730..4019870 - 141 WP_004147370.1 hypothetical protein -
IM758_RS19710 (4019891) 4019891..4020361 + 471 WP_002892026.1 YlaC family protein -
IM758_RS19715 (4020336) 4020336..4021787 - 1452 WP_023302231.1 PLP-dependent aminotransferase family protein -
IM758_RS19720 (4021888) 4021888..4022586 + 699 WP_002892021.1 GNAT family protein -
IM758_RS19725 (4022583) 4022583..4022723 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
IM758_RS19730 (4022723) 4022723..4022986 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196992 WP_002892050.1 NZ_CP071819:4018815-4019033 [Klebsiella pneumoniae subsp. pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T196992 NZ_CP095526:1565822-1566100 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCATCGACTTGCTGTGATTGATGCGGCAAGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ACCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT196992 WP_002892066.1 NZ_CP071819:4018414-4018788 [Klebsiella pneumoniae subsp. pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT196992 NZ_CP095526:1566100-1566384 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TTAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAACGCCTGGAGTCTGGCAGAGGCTGAA
AAAACGGTGGATGTGTCGCGATTACGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References