Detailed information of TA system TA196976

Bioinformatically predicted

Overview

TA module

• Type: III
• Classification (family/domain): toxIN/ToxN(toxin)
• Location: 12518..13249
• Replicon: plasmid pl86
• Accession: NZ_CP071814
• Organism: Bacillus mycoides strain JAS15/1

Toxin (Protein)

• Gene name: toxN
• Uniprot ID: -
• Locus tag: EXW36_RS30370
• Protein ID: WP_016128092.1
• Coordinates: 12677..13249 (+)
• Length: 191 a.a.

Antitoxin (RNA)

Gene name	toxI
Locus tag	-
Coordinates	12518..12614 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW36_RS30345 (7744)	7744..7953	+	210	WP_016128086.1	DUF5511 family protein	-
EXW36_RS30350 (7950)	7950..9719	+	1770	WP_016128087.1	ArdC-like ssDNA-binding domain-containing protein	-
EXW36_RS30355 (9783)	9783..10769	+	987	WP_059037856.1	hypothetical protein	-
EXW36_RS30360 (10791)	10791..11342	+	552	WP_016128090.1	metal-dependent hydrolase	-
EXW36_RS30365 (11422)	11422..12447	+	1026	WP_215567999.1	DUF3991 and TOPRIM domain-containing protein	-
- (12518)	12518..12614	+	97	NuclAT_0	-	Antitoxin
- (12518)	12518..12614	+	97	NuclAT_0	-	Antitoxin
- (12518)	12518..12614	+	97	NuclAT_0	-	Antitoxin
- (12518)	12518..12614	+	97	NuclAT_0	-	Antitoxin
EXW36_RS30370 (12677)	12677..13249	+	573	WP_016128092.1	type III toxin-antitoxin system ToxN/AbiQ family toxin	Toxin
EXW36_RS30375 (13637)	13637..15778	+	2142	WP_215568001.1	type IA DNA topoisomerase	-
EXW36_RS30380 (15808)	15808..16251	+	444	WP_016128094.1	CPBP family intramembrane metalloprotease	-
EXW36_RS30385 (16504)	16504..17373	+	870	WP_016128096.1	hypothetical protein	-
EXW36_RS30390 (17683)	17683..17916	-	234	WP_016128097.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..85331	85331

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ToxN/AbiQ toxin (8-171)

Sequences

Toxin

Protein

• Length: 191 a.a.
• Molecular weight: 22390.65 Da
• Isoelectric Point: 9.3730

>T196976 WP_016128092.1 NZ_CP071814:12677-13249 [Bacillus mycoides]
MANKDNPKFYTISTKYIDYLRETDSKVPFNKDEQHSRPYVGVLEKINGHDYFVPLTSRNDKNFNSQVSVKLFDNDNTEKR
IGVLLINNMIPVPEKECKEIDIAEKTAADPKYGNLMLKQYLFLKENMDRVSNKVEKIYKDVSVQGKPSQKQKFIKGVCCD
FPKLEEKCQEYKERDQARRIAYMRQMGRER

Nucleotide

• Length: 573 bp

>T196976 NZ_CP095520:c2637477-2637223 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAGCAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAACTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCTTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 97 bp

>AT196976 NZ_CP071814:12518-12614 [Bacillus mycoides]
GTAGCCTTACTTTAGTAGGCGCGAAAAAATTGGTGTAACTCTACCGTAGTGAGTACCAAAAAATTGGTGTAGCCTTACCG
TAGTAGGCGCAAAAAAA

Structures

Antitoxin