Detailed information of TA system TA196973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2344925..2345903
• Replicon: chromosome
• Accession: NZ_CP071808
• Organism: Bacillus mycoides strain JAS014

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: EXW53_RS12030
• Protein ID: WP_215558483.1
• Coordinates: 2344925..2345665 (+)
• Length: 247 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: R8HWP4
• Locus tag: EXW53_RS12035
• Protein ID: WP_000588712.1
• Coordinates: 2345778..2345903 (+)
• Length: 42 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EXW53_RS12005 (EXW53_12030)	2340979..2341689	+	711	WP_215558479.1	class I SAM-dependent methyltransferase	-
EXW53_RS12010 (EXW53_12035)	2341809..2342222	+	414	WP_215558480.1	VOC family protein	-
EXW53_RS12015 (EXW53_12040)	2342463..2343095	-	633	WP_215558481.1	type 1 glutamine amidotransferase family protein	-
EXW53_RS12020 (EXW53_12045)	2343371..2343847	+	477	WP_204209218.1	MarR family winged helix-turn-helix transcriptional regulator	-
EXW53_RS12025 (EXW53_12050)	2344001..2344738	+	738	WP_215558482.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
EXW53_RS12030 (EXW53_12055)	2344925..2345665	+	741	WP_215558483.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
EXW53_RS12035 (EXW53_12060)	2345778..2345903	+	126	WP_000588712.1	hypothetical protein	Antitoxin
EXW53_RS12040 (EXW53_12065)	2345979..2346155	+	177	WP_002200471.1	stage II sporulation protein SB	-
EXW53_RS12045 (EXW53_12070)	2346332..2347801	+	1470	WP_215558484.1	beta-Ala-His dipeptidase	-
EXW53_RS12050 (EXW53_12075)	2347944..2348753	+	810	WP_215558485.1	papain-like cysteine protease family protein	-
EXW53_RS12055 (EXW53_12080)	2348781..2349258	+	478	Protein_2302	hypothetical protein	-
EXW53_RS12060 (EXW53_12085)	2349625..2350806	+	1182	WP_215558486.1	agmatine deiminase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-244)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 247 a.a.
• Molecular weight: 28308.82 Da
• Isoelectric Point: 7.3943

>T196973 WP_215558483.1 NZ_CP071808:2344925-2345665 [Bacillus mycoides]
MTISNIRIGLFILAIVFIVLVFFYWKNEELYEEKKQRIRKTWYGLFITSVTVYFTIKGIDLTLWKNLLMFTAMVIFVDIA
FILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAAFGTMEWHTEEEYTKSLNAFLDSYGEKVGAK
IVVFEAVNELNTNFRGIRSQFSITVPLEHIEQLNGQQAVQVENVGIIPAKIVNDVFIVIDGKKNNLQDRDFENVYNLTIH
HSYFSK

Nucleotide

• Length: 741 bp

>T196973 NZ_CP095520:c2087233-2085911 [Escherichia coli]
ATGCCTAAACTAGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAACGGCGCGCACACCTTTAA
GTATGCAGCTGAGTGGTTAGCCAGCCGTTATGCCAGACCGTTGTCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCTAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATCCCCGAAGA
TGAAACCGTAACGTGTCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCGTTAGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCACAGGAGAAGACTGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAGGGAATAACGCCAACGACGCACATTATCAAATTGCCGATTGGCGAAAT
CAGACAGCCCAATGCGACGCTCGATCTTAGCCAAAGCGTTGATAATGAGTATTACTGTTTGCTGCTGGCGAAAGAACTCG
GGTTGAATGTTCCGGACGCAGAAATCATTAAAGCGGGAAGGGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATACTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGCCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCCGGTATCGCGCAAATTATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTCTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGGCTCACACCATTTTACGACATCATTTCGGCATTTCCGGTCCTTGGCGGTACGGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTCCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGATCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin

Protein

• Length: 42 a.a.
• Molecular weight: 4938.34 Da
• Isoelectric Point: 3.9150

>AT196973 WP_000588712.1 NZ_CP071808:2345778-2345903 [Bacillus mycoides]
VIKDYDNDFFKEDNEDTTDFSLIKGDTLQQVEELEEELKRK

Nucleotide

• Length: 126 bp

>AT196973 NZ_CP095520:c2087499-2087233 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTGGCGAATGCAATGAAACTGGTTCGCCAGCAAAACGGCTGGAC
GCAGAGCGAGTTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCTCGC
TCACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACGCGAAAAATGCCTCGCCAGAAGCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A366GQT1