Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 247558..248200 Replicon chromosome
Accession NZ_CP071808
Organism Bacillus mycoides strain JAS014

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag EXW53_RS01395 Protein ID WP_000635965.1
Coordinates 247850..248200 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag EXW53_RS01390 Protein ID WP_000004570.1
Coordinates 247558..247845 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXW53_RS01365 (EXW53_01365) 242718..243680 + 963 WP_002201996.1 UV DNA damage repair endonuclease UvsE -
EXW53_RS01370 (EXW53_01370) 243851..244399 - 549 WP_002113424.1 rhomboid family intramembrane serine protease -
EXW53_RS01375 (EXW53_01375) 244492..244851 + 360 WP_070169775.1 holo-ACP synthase -
EXW53_RS01380 (EXW53_01380) 245008..245958 + 951 WP_002201994.1 outer membrane lipoprotein carrier protein LolA -
EXW53_RS01385 (EXW53_01385) 246076..247245 + 1170 WP_002201993.1 alanine racemase -
EXW53_RS01390 (EXW53_01390) 247558..247845 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
EXW53_RS01395 (EXW53_01395) 247850..248200 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EXW53_RS01400 (EXW53_01400) 248269..250437 + 2169 WP_215558588.1 Tex family protein -
EXW53_RS01405 (EXW53_01405) 250495..250611 - 117 WP_001143642.1 cortex morphogenetic protein CmpA -
EXW53_RS01410 (EXW53_01410) 250819..251274 + 456 WP_002201991.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T196972 WP_000635965.1 NZ_CP071808:247850-248200 [Bacillus mycoides]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T196972 NZ_CP095520:c1778529-1778422 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACAACCTGGCGGCACCGATCCTGGCGGGGATTATTACCGCAGTGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT196972 WP_000004570.1 NZ_CP071808:247558-247845 [Bacillus mycoides]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT196972 NZ_CP095520:1778577-1778643 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References