Detailed information of TA system TA196968

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 461071..462314
• Replicon: chromosome
• Accession: NZ_CP071806
• Organism: Streptococcus suis strain GX69

Toxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: JZ789_RS02380
• Protein ID: WP_170244762.1
• Coordinates: 461547..462314 (+)
• Length: 256 a.a.

Antitoxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: JZ789_RS02375
• Protein ID: WP_170244761.1
• Coordinates: 461071..461547 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZ789_RS02345 (JZ789_02315)	456190..457086	-	897	WP_001809760.1	ParA family protein	-
JZ789_RS02350 (JZ789_02320)	457212..458855	+	1644	WP_230334541.1	recombinase family protein	-
JZ789_RS02355 (JZ789_02325)	458919..459995	+	1077	WP_226316946.1	toprim domain-containing protein	-
JZ789_RS02360 (JZ789_02330)	460049..460276	+	228	WP_000110921.1	DUF5965 family protein	-
JZ789_RS02365 (JZ789_02335)	460273..460662	+	390	WP_002935071.1	DUF5945 family protein	-
JZ789_RS02370 (JZ789_02340)	460712..461002	+	291	WP_044666847.1	hypothetical protein	-
JZ789_RS02375 (JZ789_02345)	461071..461547	+	477	WP_170244761.1	type II toxin-antitoxin system antitoxin PezA	Antitoxin
JZ789_RS02380 (JZ789_02350)	461547..462314	+	768	WP_170244762.1	type II toxin-antitoxin system toxin PezT	Toxin
JZ789_RS02385 (JZ789_02355)	462380..464092	+	1713	WP_170244763.1	hypothetical protein	-
JZ789_RS02390 (JZ789_02360)	464503..464862	+	360	WP_170244764.1	SAG1252 family conjugative relaxosome accessory protein	-
JZ789_RS02395 (JZ789_02365)	464872..465237	+	366	WP_018163914.1	plasmid mobilization relaxosome protein MobC	-
JZ789_RS02400 (JZ789_02370)	465224..467086	+	1863	WP_170244765.1	SAG1250 family conjugative relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	tet(O) / erm(B)	-	360173..476734	116561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (28-214)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 256 a.a.
• Molecular weight: 29025.00 Da
• Isoelectric Point: 6.5039

>T196968 WP_170244762.1 NZ_CP071806:461547-462314 [Streptococcus suis]
MRLEEFSEAEFQKALQRTIRALTRGKTIPGQPKAILLGGQSGAGKTTIHRIKQKEFQGNIIIIDGDSYRSQHPNYLALQE
KYGKDSVDYTKGFAGKMVEHLVDELSTQGYHLLIEGTLRTTQVPRQTAQLLVSKGYQVSLAVIGTKPELSYLSTLIRYEE
LYAIDPNQARVTPKEHHDGIVENLVDNLRELEREQLFDQIQIYQRDRTCIYDSAIDEGSAAEVLQECLFGKWSKVEEEMM
KEGKSSLKKLYEIRG

Nucleotide

• Length: 768 bp

>T196968 NZ_CP095520:445933-446217 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18243.65 Da
• Isoelectric Point: 4.3971

>AT196968 WP_170244761.1 NZ_CP071806:461071-461547 [Streptococcus suis]
MIGDNIKSLRRTHDLTQPEFAKMVGISRNSLSRYENGTSTVSTELIDRICQKFNVSYVDIVGEDKMLAPVEDYQLTLKVE
VIKERGAAILSQLYRYQDSQDIAFDDESNPWILMSDDLAELINTKIYLVDTFDEIERYNGYLDGIERMLDMVHHRVVA

Nucleotide

• Length: 477 bp

>AT196968 NZ_CP095520:445697-445930 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG