Detailed information of TA system TA196952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 974621..975244
• Replicon: chromosome
• Accession: NZ_CP071802
• Organism: Yersinia ruckeri strain KMM821

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: YR821_RS04400
• Protein ID: WP_049687933.1
• Coordinates: 974621..974824 (-)
• Length: 68 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: A0A085U5C5
• Locus tag: YR821_RS04405
• Protein ID: WP_004718732.1
• Coordinates: 974876..975244 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YR821_RS04375 (YR821_0892)	970476..970814	+	339	WP_007886947.1	P-II family nitrogen regulator	-
YR821_RS04380 (YR821_0893)	970853..972142	+	1290	WP_004718723.1	ammonium transporter AmtB	-
YR821_RS04385 (YR821_0894)	972257..973120	-	864	WP_004718726.1	acyl-CoA thioesterase II	-
YR821_RS04390 (YR821_0895)	973498..973812	-	315	WP_004718728.1	MGMT family protein	-
YR821_RS04400 (YR821_0897)	974621..974824	-	204	WP_049687933.1	expression modulating protein YmoA	Toxin
YR821_RS04405 (YR821_0898)	974876..975244	-	369	WP_004718732.1	Hha toxicity modulator TomB	Antitoxin
YR821_RS04410 (YR821_0900)	976293..976454	+	162	WP_004718735.1	hypothetical protein	-
YR821_RS04415 (YR821_0901)	976573..976716	-	144	WP_004718738.1	type B 50S ribosomal protein L36	-
YR821_RS04420 (YR821_0902)	976736..976990	-	255	WP_038243728.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (8-64)

Antitoxin

• Biofilm formation regulator YbaJ (1-117)

Sequences

Toxin

Protein

• Length: 68 a.a.
• Molecular weight: 8019.24 Da
• Isoelectric Point: 6.4573

>T196952 WP_049687933.1 NZ_CP071802:c974824-974621 [Yersinia ruckeri]
MTKTDYLIRLRKCTTIDTLERVIEKNKYELSDDELELFYSAADHRLAELTMNKLYDKIPATVWQHVK

Nucleotide

• Length: 204 bp

>T196952 NZ_CP095518:2993943-2994320 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGCTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCACTGAACTTTCTCGTTGAAAAATACGCCCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCTGCTCTCAGTTAATCAACAGTATCGATATCCTCCGGGCACGCCGGGCAACCGGCCTGATGACACGCGA
CAACTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 14277.08 Da
• Isoelectric Point: 4.4225

>AT196952 WP_004718732.1 NZ_CP071802:c975244-974876 [Yersinia ruckeri]
MDEYSPKRHDIAQLKFLCENLYDEGIATLGDSHHGWVNDPTSAVNLQLNDLIEHIASFVMSFKIKYADEIDLCELVEEYL
DDTYTLFSSYGINDPDLRRWQKTKDRLFRLFSGEYLCALMKN

Nucleotide

• Length: 369 bp

>AT196952 NZ_CP095518:2993485-2993853 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATCACCCCGACGGCAATAACAGCAGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
ACCCTGTTTTGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGATGCAGATGCATGCCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAACAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A085U5C5