Detailed information of TA system TA196946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 2013661..2014700
• Replicon: chromosome
• Accession: NZ_CP071797
• Organism: Pseudomonas fluorescens strain YK-310

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: JZM58_RS09155
• Protein ID: WP_207985434.1
• Coordinates: 2013661..2014236 (-)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: J2X4E7
• Locus tag: JZM58_RS09160
• Protein ID: WP_007959254.1
• Coordinates: 2014233..2014700 (-)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZM58_RS09130 (JZM58_09130)	2008988..2010976	+	1989	WP_207985431.1	heme lyase CcmF/NrfE family subunit	-
JZM58_RS09135 (JZM58_09135)	2010973..2011509	+	537	WP_085611514.1	DsbE family thiol:disulfide interchange protein	-
JZM58_RS09140 (JZM58_09140)	2011506..2011976	+	471	WP_064378637.1	cytochrome c-type biogenesis protein CcmH	-
JZM58_RS09145 (JZM58_09145)	2011973..2013175	+	1203	WP_207985432.1	c-type cytochrome biogenesis protein CcmI	-
JZM58_RS09150 (JZM58_09150)	2013201..2013608	+	408	WP_207985433.1	hypothetical protein	-
JZM58_RS09155 (JZM58_09155)	2013661..2014236	-	576	WP_207985434.1	PIN domain-containing protein	Toxin
JZM58_RS09160 (JZM58_09160)	2014233..2014700	-	468	WP_007959254.1	helix-turn-helix domain-containing protein	Antitoxin
JZM58_RS09165 (JZM58_09165)	2014785..2015672	-	888	WP_207985435.1	LysR family transcriptional regulator	-
JZM58_RS09170 (JZM58_09170)	2015743..2016930	-	1188	WP_207985436.1	MFS transporter	-
JZM58_RS09175 (JZM58_09175)	2017172..2017792	+	621	WP_207985437.1	DUF5666 domain-containing protein	-
JZM58_RS09180 (JZM58_09180)	2017849..2018847	-	999	WP_127797317.1	sulfate ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (8-115)

Antitoxin

• SinI-like, DNA-binding domain (84-130)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21587.92 Da
• Isoelectric Point: 5.9178

>T196946 WP_207985434.1 NZ_CP071797:c2014236-2013661 [Pseudomonas fluorescens]
VRHSSFTVLYDACVLYPAPLRDFLMWLALSGRFRARWTQEIHCEWKRNLLKNRPDLTMEQLDRTSSLMDQAIPDACVHDY
EDLIAGLTLPDCDDRHVLAAAIRCGASVIVTFNQKDFPCKALEPFGIEAQHPDEFVDNLLGLDPAAVVAAAQKQRRQLKM
PAMDVEPFLDLLQRQGLIKSVQALSGYHAIL

Nucleotide

• Length: 576 bp

>T196946 NZ_CP095518:1214242-1214394 [Escherichia coli]
ATGAAGCAGCATAAGGCGATGATTGTCGCCCTGATCGTCATCTGTATCACCGCCGTAGTGGCGGCGCTGGTAACGAGAAA
AGACCTCTGTGAGGTTCACATCCGAACTGGCCAGACGGAGGTTGCTGTTTTCACGGCTTACGAATCCGAGTAA

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17272.70 Da
• Isoelectric Point: 6.4962

>AT196946 WP_007959254.1 NZ_CP071797:c2014700-2014233 [Pseudomonas fluorescens]
MSTVIHPSISLPVEREVKAAIEGQRALAAYLATHFETQHIQIFDEQNEAHRVELPTSALRLLIDILAELAAGNAVQVVPV
HAELTTQEAADLLNVSRPHLIKLLESGELSYHKTGKHRRVRFADLMDYKTRRTTASEQAMALLAEQAQALRTGYE

Nucleotide

• Length: 468 bp

>AT196946 NZ_CP095518:c1214193-1214136 [Escherichia coli]
GTTCTGCATATAGGGGGCCTCGGGTTGATGGTAAAATATCACTCGGGGCTTTTCTCTA

Structures

Antitoxin

• AlphaFold DB: J2X4E7