Detailed information of TA system TA196939

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 2444785..2445515
• Replicon: chromosome
• Accession: NZ_CP071796
• Organism: Ottowia testudinis strain 27C

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J1M35_RS11475
• Protein ID: WP_208007186.1
• Coordinates: 2445081..2445515 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J1M35_RS11470
• Protein ID: WP_208007185.1
• Coordinates: 2444785..2445084 (+)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1M35_RS11450 (J1M35_11450)	2439894..2441225	+	1332	WP_208007182.1	hypothetical protein	-
J1M35_RS11455 (J1M35_11455)	2441590..2442572	+	983	Protein_2276	IS110 family transposase	-
J1M35_RS11460 (J1M35_11460)	2442675..2444201	-	1527	WP_208007183.1	rhomboid family intramembrane serine protease	-
J1M35_RS11465 (J1M35_11465)	2444341..2444697	-	357	WP_208007184.1	heavy metal-binding domain-containing protein	-
J1M35_RS11470 (J1M35_11470)	2444785..2445084	+	300	WP_208007185.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J1M35_RS11475 (J1M35_11475)	2445081..2445515	+	435	WP_208007186.1	PIN domain-containing protein	Toxin
J1M35_RS11480 (J1M35_11480)	2445942..2446565	+	624	WP_208007187.1	IPTL-CTERM sorting domain-containing protein	-
J1M35_RS11485 (J1M35_11485)	2446589..2447571	-	983	Protein_2282	IS110 family transposase	-
J1M35_RS11490 (J1M35_11490)	2448069..2448617	-	549	WP_208007188.1	transposase	-
J1M35_RS11495 (J1M35_11495)	2448731..2448883	-	153	WP_208007189.1	PLP-dependent transferase	-
J1M35_RS11500 (J1M35_11500)	2448947..2450245	-	1299	WP_208007190.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-128)

Antitoxin

• SpoVT-AbrB domain (20-56)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15250.67 Da
• Isoelectric Point: 6.7168

>T196939 WP_208007186.1 NZ_CP071796:2445081-2445515 [Ottowia testudinis]
MSALIYLLDTNIVSDAMRNPFGPVAQRILGRPADDGPALGISVVVECELQFGLARKSSPRLQAAYEPLRAALRVWPLDDA
VPPHYAALRAHLETQGTPIGLNNALIAAHALALGATLVSGDAEFARVPGLKVENWLKPLATPGV

Nucleotide

• Length: 435 bp

>T196939 NZ_CP095514:5027762-5028187 [Escherichia coli]
ATGAAAATTATTCCTGCAACGACGCTGCGGGCGACGACGTCATATCTGTCTCCTGTCGTGGTCTGGCAAACATTACTGGC
CCGCCTGCTGGAGCAGCACTACGGGCTGAATCTTAATGACACACCATTCAACAATGAAAAGGTGATTCAGGAACACATAG
ACGCCGGGATCACTCTGGTCAATGCCGTCAATTTTCTGGTGGAGAAGTACGAATTGGTCCGTATCGACAGGAAAGGATTT
AGCTGGCAGGAACAGACGCCTTATCTTCGTGCGGTCGATATTTTGCGGGCGCGGCAGGCGACCGGCCTGCGGATTCAGTG
CAAAAACACGAATATAATCAGCCGGATGGCTGATTCAAGACATGATAAAAGACTTTACCAGCGCACGTTTAAAGCGTGCA
AGAGATGTTATTATACTAAAACATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10872.35 Da
• Isoelectric Point: 4.2296

>AT196939 WP_208007185.1 NZ_CP071796:2444785-2445084 [Ottowia testudinis]
MSAVPNDLQPAAAPRRVKFFRNGANQAVRIPREFELPGDEATVRREGDALIIEPVEPVYPKGSVAALQQALRKLQALGPI
DEEFPDVDEGLLPLDDIEL

Nucleotide

• Length: 300 bp

>AT196939 NZ_CP095514:5027396-5027731 [Escherichia coli]
ATGCTGCAAAAGGACAAGGTCATGCAACAAGAATGGGGGCTTCCCTGTACCGTAACACCACGCTTCACTGCTCGACTGGT
ACAGGAAAGACATAACCTGCATTACCTGGCTGATCGGGCCGCAATCACCGGGAAATTCAGCTCCCCGGATCTGCTGCATA
TCGACCAGGCTTTTCCGGTGTTGATGAAACAACTGGAGCTGAAACTGGCTGACGGTGAACTCTCCCCCTGCCAACAACGT
ACCGTCACGCTATACGCCAAAGGACTAACCTGCGAAGCCGATACGCTGGGTTCCTGTGGTTACGTCTATATCGCTATTTA
TCCCACTCAACGCTGA