Detailed information of TA system TA196935

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 1552720..1553507
• Replicon: chromosome
• Accession: NZ_CP071796
• Organism: Ottowia testudinis strain 27C

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: J1M35_RS07220
• Protein ID: WP_208010555.1
• Coordinates: 1552720..1553214 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: J1M35_RS07225
• Protein ID: WP_208010556.1
• Coordinates: 1553211..1553507 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1M35_RS07195 (J1M35_07195)	1548296..1549462	-	1167	WP_208010551.1	efflux RND transporter periplasmic adaptor subunit	-
J1M35_RS07200 (J1M35_07200)	1549473..1550126	-	654	WP_208010552.1	TetR/AcrR family transcriptional regulator	-
J1M35_RS07205 (J1M35_07205)	1550476..1551534	-	1059	WP_208011229.1	serine/threonine protein kinase	-
J1M35_RS07210 (J1M35_07210)	1551563..1552048	+	486	WP_208010553.1	EVE domain-containing protein	-
J1M35_RS07215 (J1M35_07215)	1552035..1552562	+	528	WP_208010554.1	GNAT family N-acetyltransferase	-
J1M35_RS07220 (J1M35_07220)	1552720..1553214	-	495	WP_208010555.1	GNAT family N-acetyltransferase	Toxin
J1M35_RS07225 (J1M35_07225)	1553211..1553507	-	297	WP_208010556.1	DUF1778 domain-containing protein	Antitoxin
J1M35_RS07230 (J1M35_07230)	1553694..1554161	+	468	WP_208010557.1	DUF1203 domain-containing protein	-
J1M35_RS07235 (J1M35_07235)	1554219..1555403	-	1185	WP_208010558.1	acyl-CoA dehydrogenase	-
J1M35_RS07240 (J1M35_07240)	1555507..1556403	+	897	WP_208010559.1	LysR family transcriptional regulator	-
J1M35_RS07245 (J1M35_07245)	1556428..1556967	+	540	WP_208010560.1	peptide-methionine (S)-S-oxide reductase MsrA	-
J1M35_RS07250 (J1M35_07250)	1557107..1557544	+	438	WP_208010561.1	CopD family protein	-
J1M35_RS07255 (J1M35_07255)	1557573..1558205	-	633	WP_208010562.1	uracil phosphoribosyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (43-145)

Antitoxin

• Vibrio phage ICP1, Orf50 (15-91)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18000.80 Da
• Isoelectric Point: 9.2096

>T196935 WP_208010555.1 NZ_CP071796:c1553214-1552720 [Ottowia testudinis]
MSLSAPGALQASHICQGFDCGRPALNDWLLRHALQAQASGSARSFVVYNDARVAAYCSLTVGQIDTLDAPERVRRGMGQY
PIPVMVLARLAVDKAWQGQDLGVGLLQDAIRRTLLVAAQAGIRALLTHPLDDAAEAFYRRFGFEPTLVRERQLLLLLKDA
RRFA

Nucleotide

• Length: 495 bp

>T196935 NZ_CP095514:c3982130-3981723 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10520.93 Da
• Isoelectric Point: 4.7247

>AT196935 WP_208010556.1 NZ_CP071796:c1553507-1553211 [Ottowia testudinis]
MSTLALSAPAGRSARLGLRATPEQEGVLRRAAEVAQKSLTDFVLDAACQAAEQTLLDQRLFLVSGAQYQSLLELLDRPEG
DNPGLADLFARRPPWSAA

Nucleotide

• Length: 297 bp

>AT196935 NZ_CP095514:c3982377-3982111 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTTTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA