Detailed information of TA system TA196921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 3542491..3543140
• Replicon: chromosome
• Accession: NZ_CP071780
• Organism: Proteus mirabilis strain swupm3

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: J3U94_RS16220
• Protein ID: WP_049256512.1
• Coordinates: 3542491..3542910 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: B4EZC0
• Locus tag: J3U94_RS16225
• Protein ID: WP_012368535.1
• Coordinates: 3542907..3543140 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3U94_RS16190 (J3U94_16160)	3537768..3538646	-	879	WP_049203255.1	23S rRNA pseudouridine(2604) synthase RluF	-
J3U94_RS16195 (J3U94_16165)	3538822..3539736	-	915	WP_004249085.1	fatty acid biosynthesis protein FabY	-
J3U94_RS16200 (J3U94_16170)	3539760..3540197	-	438	WP_071233548.1	D-aminoacyl-tRNA deacylase	-
J3U94_RS16205 (J3U94_16175)	3540278..3540895	-	618	WP_020946398.1	glucose-1-phosphatase	-
J3U94_RS16210 (J3U94_16180)	3541549..3541965	-	417	WP_049208252.1	hypothetical protein	-
J3U94_RS16215 (J3U94_16185)	3541943..3542266	-	324	WP_049208253.1	hypothetical protein	-
J3U94_RS16220 (J3U94_16190)	3542491..3542910	-	420	WP_049256512.1	type II toxin-antitoxin system VapC family toxin	Toxin
J3U94_RS16225 (J3U94_16195)	3542907..3543140	-	234	WP_012368535.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
J3U94_RS16230 (J3U94_16200)	3543891..3545726	-	1836	WP_004246802.1	ribosome-dependent GTPase TypA	-
J3U94_RS16235 (J3U94_16205)	3546086..3547495	+	1410	WP_004246801.1	glutamate--ammonia ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (9-56)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 15401.91 Da
• Isoelectric Point: 7.7288

>T196921 WP_049256512.1 NZ_CP071780:c3542910-3542491 [Proteus mirabilis]
VKKVYMLDTNICSFIMREQPISLLEKLQKCVMNHDTIVISTITYSEMRFGAIGKKASPKHNRLVDAFCERVDAILAWDKA
AVDATTVIKKCLSDVGLPIGNNDSAIAGHAVAVNAILVTNNTREFSRVEGLKIEDWTHV

Nucleotide

• Length: 420 bp

>T196921 NZ_CP095514:735167-735544 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCAGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGTACCGACCTGCCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGATCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8890.06 Da
• Isoelectric Point: 4.4870

>AT196921 WP_012368535.1 NZ_CP071780:c3543140-3542907 [Proteus mirabilis]
MRTRTVAVFKNGSNRAIRLPKDFDFDGVNELEISRDGDCIILRPVKPNWISLQNEEKADSDFLLERDDVIEEGRVIL

Nucleotide

• Length: 234 bp

>AT196921 NZ_CP095514:734709-735077 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: B4EZC0