Detailed information of TA system TA196920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-MqsA
• Location: 3521921..3522620
• Replicon: chromosome
• Accession: NZ_CP071780
• Organism: Proteus mirabilis strain swupm3

Toxin (Protein)

• Gene name: relE
• Uniprot ID: B4EZ96
• Locus tag: J3U94_RS16115
• Protein ID: WP_004249093.1
• Coordinates: 3521921..3522307 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: B4EZ97
• Locus tag: J3U94_RS16120
• Protein ID: WP_004246828.1
• Coordinates: 3522300..3522620 (+)
• Length: 107 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3U94_RS16100 (J3U94_16070)	3517940..3518521	-	582	WP_004246833.1	DNA-3-methyladenine glycosylase I	-
J3U94_RS16105 (J3U94_16075)	3518772..3519677	+	906	WP_004246832.1	glycine--tRNA ligase subunit alpha	-
J3U94_RS16110 (J3U94_16080)	3519687..3521759	+	2073	WP_041701373.1	glycine--tRNA ligase subunit beta	-
J3U94_RS16115 (J3U94_16085)	3521921..3522307	+	387	WP_004249093.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J3U94_RS16120 (J3U94_16090)	3522300..3522620	+	321	WP_004246828.1	helix-turn-helix domain-containing protein	Antitoxin
J3U94_RS16125 (J3U94_16095)	3522669..3523103	-	435	WP_020946391.1	hypothetical protein	-
J3U94_RS16130 (J3U94_16100)	3523096..3523980	-	885	WP_012368526.1	endonuclease/exonuclease/phosphatase family protein	-
J3U94_RS16135 (J3U94_16105)	3524198..3525484	-	1287	WP_253343077.1	DUF3748 domain-containing protein	-
J3U94_RS16140 (J3U94_16110)	3525621..3526238	+	618	WP_004246822.1	trimeric intracellular cation channel family protein	-
J3U94_RS16145 (J3U94_16115)	3526540..3527163	+	624	WP_004246821.1	guanylate kinase	-
J3U94_RS16150 (J3U94_16120)	3527218..3527493	+	276	WP_004246820.1	DNA-directed RNA polymerase subunit omega	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-125)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-95)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14294.64 Da
• Isoelectric Point: 10.0642

>T196920 WP_004249093.1 NZ_CP071780:3521921-3522307 [Proteus mirabilis]
MVIYKTKMFKNSFKKITLTNAELIAIATEVLAGQFEADLGGGVIKKRACIQGKGKSSGIRTIIFYKQGNNLFFADGWKKS
SLSSKKTKEITDDELESYKDLAKDLFNANQNKIDKMIALGLLTEVKYD

Nucleotide

• Length: 387 bp

>T196920 NZ_CP095514:547019-547192 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTTCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 107 a.a.
• Molecular weight: 12126.21 Da
• Isoelectric Point: 10.4411

>AT196920 WP_004246828.1 NZ_CP071780:3522300-3522620 [Proteus mirabilis]
MTDKMKEMLKFAQEMEDAGVTDGSTTKRIAARIKAKELQAKYKHPTPMTGEDIKRIREQNNISQSSLALILNMTKESVSK
WERNEIKPNGAALRLLHLIDKKILFI

Nucleotide

• Length: 321 bp

>AT196920 NZ_CP095514:546695-546991 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: B4EZ96

Antitoxin

• AlphaFold DB: B4EZ97