Detailed information of TA system TA196919

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3057550..3058571
• Replicon: chromosome
• Accession: NZ_CP071780
• Organism: Proteus mirabilis strain swupm3

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: J3U94_RS14130
• Protein ID: WP_004249488.1
• Coordinates: 3058002..3058571 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: J3U94_RS14125
• Protein ID: WP_004245528.1
• Coordinates: 3057550..3058002 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3U94_RS14105 (J3U94_14080)	3053336..3053866	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
J3U94_RS14110 (J3U94_14085)	3054183..3055148	+	966	WP_253343386.1	thiamine ABC transporter substrate binding subunit	-
J3U94_RS14115 (J3U94_14090)	3055148..3056728	+	1581	WP_046334723.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
J3U94_RS14120 (J3U94_14095)	3056721..3057422	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
J3U94_RS14125 (J3U94_14100)	3057550..3058002	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
J3U94_RS14130 (J3U94_14105)	3058002..3058571	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
J3U94_RS14135 (J3U94_14110)	3058687..3061041	+	2355	WP_004249486.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T196919 WP_004249488.1 NZ_CP071780:3058002-3058571 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T196919 NZ_CP095514:239190-239501 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT196919 WP_004245528.1 NZ_CP071780:3057550-3058002 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT196919 NZ_CP095514:239502-239792 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Antitoxin

• AlphaFold DB: B4F2H3