Detailed information of TA system TA196909

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 3057565..3058586
• Replicon: chromosome
• Accession: NZ_CP071773
• Organism: Proteus mirabilis strain swupm1

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: J3U92_RS14095
• Protein ID: WP_004249488.1
• Coordinates: 3058017..3058586 (+)
• Length: 190 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: B4F2H3
• Locus tag: J3U92_RS14090
• Protein ID: WP_004245528.1
• Coordinates: 3057565..3058017 (+)
• Length: 151 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J3U92_RS14070 (J3U92_14050)	3053351..3053881	-	531	WP_004245524.1	RNA pyrophosphohydrolase	-
J3U92_RS14075 (J3U92_14055)	3054198..3055163	+	966	WP_253343386.1	thiamine ABC transporter substrate binding subunit	-
J3U92_RS14080 (J3U92_14060)	3055163..3056743	+	1581	WP_046334723.1	thiamine/thiamine pyrophosphate ABC transporter permease ThiP	-
J3U92_RS14085 (J3U92_14065)	3056736..3057437	+	702	WP_004245527.1	thiamine ABC transporter ATP-binding protein ThiQ	-
J3U92_RS14090 (J3U92_14070)	3057565..3058017	+	453	WP_004245528.1	helix-turn-helix domain-containing protein	Antitoxin
J3U92_RS14095 (J3U92_14075)	3058017..3058586	+	570	WP_004249488.1	PIN domain-containing protein	Toxin
J3U92_RS14100 (J3U92_14080)	3058702..3061056	+	2355	WP_004249486.1	DNA polymerase II	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-113)

Antitoxin

• SinI-like, DNA-binding domain (80-125)

Sequences

Toxin

Protein

• Length: 190 a.a.
• Molecular weight: 21600.10 Da
• Isoelectric Point: 7.3470

>T196909 WP_004249488.1 NZ_CP071773:3058017-3058586 [Proteus mirabilis]
MTNYTVLLDACVMYPAPLRSYLMYLANTGLFRARWSNQIHDEWITNLLVNNPTLTAERLIRTRDLMNAHVPDCLVEGYEP
LICGLKLPDPNDRHVLAAAIKGQAQAIITFNLKDFPQKYLNSFDISAIHPDEFLSDMFELNPSSCIKAAQQHRQSLKNPP
MTVETFLGCLQKQKLPQFISHLRAFQILL

Nucleotide

• Length: 570 bp

>T196909 NZ_CP095510:3307518-3308012 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 151 a.a.
• Molecular weight: 16700.15 Da
• Isoelectric Point: 5.7723

>AT196909 WP_004245528.1 NZ_CP071773:3057565-3058017 [Proteus mirabilis]
MNTISERLSAEDIALAKLSSQELSAVMDINGKTQKLSIIDKTGNNHIVQIPNSALRLMIEILTQLGQGNTVSITPIHAEL
TTQEAADILNMSRPTFVKLLDTRAIPYSRKGNRRKVAYADIVAYKNELEQKRLDALAELSQLDQEMDMGY

Nucleotide

• Length: 453 bp

>AT196909 NZ_CP095510:3307228-3307521 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Antitoxin

• AlphaFold DB: B4F2H3