Detailed information of TA system TA196907

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 74176..74764
• Replicon: plasmid pGX7
• Accession: NZ_CP071772
• Organism: Acinetobacter towneri strain GX7

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: J4G46_RS14040
• Protein ID: WP_004647749.1
• Coordinates: 74468..74764 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J4G46_RS14035
• Protein ID: WP_004969304.1
• Coordinates: 74176..74466 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J4G46_RS14005 (J4G46_14005)	69745..70341	+	597	WP_074163998.1	LysE family transporter	-
J4G46_RS14010 (J4G46_14010)	70369..71187	-	819	WP_032073151.1	AraC family transcriptional regulator	-
J4G46_RS14015 (J4G46_14015)	71318..71599	-	282	WP_000985610.1	putative addiction module antidote protein	-
J4G46_RS14020 (J4G46_14020)	71600..71902	-	303	WP_154320144.1	type II toxin-antitoxin system RelE/ParE family toxin	-
J4G46_RS14025 (J4G46_14025)	72152..72361	+	210	WP_004976362.1	hypothetical protein	-
J4G46_RS14030 (J4G46_14030)	72611..74065	+	1455	WP_026438532.1	SulP family inorganic anion transporter	-
J4G46_RS14035 (J4G46_14035)	74176..74466	-	291	WP_004969304.1	putative addiction module antidote protein	Antitoxin
J4G46_RS14040 (J4G46_14040)	74468..74764	-	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J4G46_RS14655	74981..75106	+	126	WP_004969301.1	hypothetical protein	-
J4G46_RS14045 (J4G46_14045)	75200..76282	-	1083	WP_004969299.1	hypothetical protein	-
J4G46_RS14050 (J4G46_14050)	76451..77776	+	1326	WP_179989167.1	ISNCY family transposase	-
J4G46_RS14055 (J4G46_14055)	78243..78809	+	567	WP_006581699.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(E) / msr(E) / erm(B) / blaOXA-58 / floR / sul2 / tet(X3) / aph(3')-Ia	-	1..178739	178739

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T196907 WP_004647749.1 NZ_CP071772:c74764-74468 [Acinetobacter towneri]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T196907 NZ_CP095510:c2911587-2911411 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTGGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCAAGGGCGACGCAGTGTCATGCCGCGTCACCCCAGTGATGAGATAAAAGAACCATTGCGTAAAGCAATCCTGAAGC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10524.19 Da
• Isoelectric Point: 5.1133

>AT196907 WP_004969304.1 NZ_CP071772:c74466-74176 [Acinetobacter towneri]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSAPRFDTI
NRVVNALGLKLTVQHA

Nucleotide

• Length: 291 bp

>AT196907 NZ_CP095510:c2911365-2910949 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ATGAGCTAATCCCGTTGCCTTCGCCATTAACTAACAACGATCACTTTATTGAAGTGCCTTTAAGTATCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA