Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 35096..35624 Replicon plasmid pGX7
Accession NZ_CP071772
Organism Acinetobacter towneri strain GX7

Toxin (Protein)


Gene name relE Uniprot ID N8SCV7
Locus tag J4G46_RS13835 Protein ID WP_004733298.1
Coordinates 35096..35389 (-) Length 98 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A1E8E1G7
Locus tag J4G46_RS13840 Protein ID WP_070154357.1
Coordinates 35379..35624 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J4G46_RS13790 (J4G46_13790) 30313..30627 - 315 WP_104505827.1 helix-turn-helix domain-containing protein -
J4G46_RS13795 (J4G46_13795) 30608..30931 - 324 WP_104505828.1 transcriptional regulator -
J4G46_RS13800 (J4G46_13800) 31011..31115 + 105 Protein_33 transposase domain-containing protein -
J4G46_RS13805 (J4G46_13805) 31243..31782 - 540 WP_265738119.1 IS630 family transposase -
J4G46_RS13810 (J4G46_13810) 31799..32254 - 456 WP_004973749.1 helix-turn-helix domain-containing protein -
J4G46_RS13815 (J4G46_13815) 32330..32743 + 414 WP_179989130.1 hypothetical protein -
J4G46_RS13820 (J4G46_13820) 32746..33060 + 315 WP_070155405.1 hypothetical protein -
J4G46_RS13825 (J4G46_13825) 33231..34557 - 1327 Protein_38 ISNCY family transposase -
J4G46_RS13830 (J4G46_13830) 34702..35004 - 303 WP_136144098.1 hypothetical protein -
J4G46_RS13835 (J4G46_13835) 35096..35389 - 294 WP_004733298.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J4G46_RS13840 (J4G46_13840) 35379..35624 - 246 WP_070154357.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
J4G46_RS13845 (J4G46_13845) 36117..36539 - 423 WP_004676061.1 hypothetical protein -
J4G46_RS13850 (J4G46_13850) 36643..36966 + 324 WP_004676058.1 hypothetical protein -
J4G46_RS13855 (J4G46_13855) 37142..37753 + 612 WP_163146741.1 recombinase family protein -
J4G46_RS13860 (J4G46_13860) 37943..38827 - 885 WP_000155092.1 Mph(E) family macrolide 2'-phosphotransferase -
J4G46_RS13865 (J4G46_13865) 38883..40358 - 1476 WP_000052512.1 ABC-F type ribosomal protection protein Msr(E) -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mph(E) / msr(E) / erm(B) / blaOXA-58 / floR / sul2 / tet(X3) / aph(3')-Ia - 1..178739 178739
- inside IScluster/Tn mph(E) / msr(E) / erm(B) - 18864..42913 24049


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(8-84)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-71)


Sequences

Toxin        


Download         Length: 98 a.a.        Molecular weight: 11252.46 Da        Isoelectric Point: 10.4456

>T196905 WP_004733298.1 NZ_CP071772:c35389-35096 [Acinetobacter towneri]
MTYKLLRHKDFTAEWEKLPSAIRDQFKKKLAKVIEQPHIPKNMLKGDLAGCYKIKLLKAGVRLVYQVKDDQVVILLITVG
KRADSIVYDEAKKRIKD

Download         Length: 294 bp

>T196905 NZ_CP095510:2810281-2811603 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
CGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGAAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGCTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACCGTCGCTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9204.56 Da        Isoelectric Point: 4.2703

>AT196905 WP_070154357.1 NZ_CP071772:c35624-35379 [Acinetobacter towneri]
MATVNFRVDEALKEKSYSILKEQGIAPTEFFTNILEYIATTGKLPVQKALLSEEDADLLALVRKRVNDPKEMFEEVTLDD
L

Download         Length: 246 bp

>AT196905 NZ_CP095510:2810015-2810281 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGTATTAAGCAGGCGACAATTTCCAATTTCGAAAACAATCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB N8SCV7

Antitoxin

Source ID Structure
AlphaFold DB A0A1E8E1G7

References